Anticorps Polyclonal de lapin anti-IWS1

• Phare
• Validé par KD/KO

IWS1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (2)

N° de cat : 16943-1-AP

Synonymes

IWS1, IWS1 homolog (S. cerevisiae), IWS1 like protein, IWS1L, Protein IWS1 homolog

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Galerie de données de validation

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  WB analysis using 16943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16943-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16943-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 16943-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16943-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16943-1-AP (IWS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16943-1-AP

  IP result of anti-IWS1 (IP:16943-1-AP, 4ug; Detection:16943-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse lung using 16943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16943-1-AP (IWS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 16943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16943-1-AP (IWS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 16943-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using IWS1 antibody (16943-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu pulmonaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• ChIP: See 1 publications below

Informations sur le produit

16943-1-AP cible IWS1 dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IWS1 Protéine recombinante Ag10554
• Nom complet: IWS1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 819 aa, 92 kDa
• Poids moléculaire observé: 116-120 kDa
• Numéro d’acquisition GenBank: BC017012
• Symbole du gène: IWS1
• Identification du gène (NCBI): 55677
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IWS1, as a transcription elongation factor, functions to recruit cofactors to the RNA polymerase II (RNAPII) complex to regulate mRNA alternative splicing (PMID: 30846735; 37237004). It plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. It also acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription (PMID: 17184735; 17234882; 19141475).

Publications

Species	Application	Title
	ChIP	Cell Host Microbe; The Integrase Cofactor LEDGF/p75 Associates with Iws1 and Spt6 for Postintegration Silencing of HIV-1 Gene Expression in Latently Infected Cells.; Authors - Annabelle Gérard

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Fabienne (Verified Customer) (06-12-2024)

Works great in western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa, HEK293T and Jurkat cell lines

IWS1 Antibody Western Blot validation (1:1000 dilution) in HeLa, HEK293T and Jurkat cell lines (Cat no:16943-1-AP)

FH

Sarah (Verified Customer) (12-31-2020)

Works perfectly in WB and immunoprecipitation experiements (HeLa and HEL293T cells).

• Applications: Western Blot, Immunoprecipitation, ChIP
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa and HEK293T