Anticorps Polyclonal de lapin anti-IVD

IVD Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 10822-1-AP

Synonymes

ACAD2, IVD

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Galerie de données de validation

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  WB analysis using 10822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10822-1-AP (IVD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10822-1-AP (IVD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10822-1-AP (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10822-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10822-1-AP (IVD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10822-1-AP

  IP result of anti-IVD (IP:10822-1-AP, 4ug; Detection:10822-1-AP 1:300) with MCF-7 cells lysate 800ug.

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  IHC staining of human liver cancer using 10822-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10822-1-AP (IVD antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10822-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10822-1-AP (IVD antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10822-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10822-1-AP (IVD antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10822-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10822-1-AP (IVD antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10822-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 10822-1-AP (IVD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HepG2, tissu cérébral humain, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below

Informations sur le produit

10822-1-AP cible IVD dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris, arabidopsis
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IVD Protéine recombinante Ag1252
• Nom complet: isovaleryl Coenzyme A dehydrogenase
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 41-46 kDa
• Numéro d’acquisition GenBank: BC017202
• Symbole du gène: IVD
• Identification du gène (NCBI): 3712
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD(PMID:2063866). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
Arabidopsis	WB	Plant J; PMID: 19929878; Authors - Gu Liping L
mouse	WB	Cell Mol Life Sci; Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.; Authors - Min-Zhi Peng
mouse	WB	Mol Endocrinol; Research resource: A dual proteomic approach identifies regulated islet proteins during beta cell mass expansion in vivo.; Authors - Signe Horn
mouse	WB	J Neurochem; Proteomic analysis of stargazer mutant mouse neuronal proteins involved in absence seizure.; Authors - Ryu Myung-Jeom MJ
	WB	Plant Cell Rep; The role of the electron-transfer flavoprotein: ubiquinone oxidoreductase following carbohydrate starvation in Arabidopsis cell cultures.; Authors - Danielle S Brito
rat	WB	Proteomics Clin Appl; Loss of Pink1 modulates synaptic mitochondrial bioenergetics in the rat striatum prior to motor symptoms: concomitant complex I respiratory defects and increased complex II-mediated respiration.; Authors - Kelly L Stauch

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lana (Verified Customer) (06-21-2019)

Lines on WB:1 - BioRad Precision Plus Protein standards2 and 3 – biological replicates, lysates of mitochondrial fractions of ST-Hdh-Q7/Q7 mouse striatal cell line.SDS-PAGE:15 ug/ul RIPA lysate of the mitochondrial fraction of ST-Hdh-Q7/Q7 mouse striatal cell line4-12% Bis-tris gradient gelTransfer:Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hPrimary Ab:O/N incubation at 4CSecondary Ab:IRDye 680LT Goat anti-Rabbit, 1h incubation at room temperature.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ST-Hdh mouse striatal cell line

IVD Antibody Western Blot, validation (1:1000 dilution) in ST-Hdh mouse striatal cell line (Cat no:10822-1-AP)