Anticorps Polyclonal de lapin anti-ITCH

• Phare
• Validé par KD/KO

ITCH Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 20920-1-AP

Synonymes

AIF4, AIP4, ITCH, NAPP1, NFE2 associated polypeptide 1

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Galerie de données de validation

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  WB analysis using 20920-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20920-1-AP (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 20920-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 20920-1-AP (ITCH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 20920-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20920-1-AP (ITCH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20920-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20920-1-AP (ITCH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20920-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20920-1-AP (ITCH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20920-1-AP

  HeLa lysates prepared and IP of ITCH performed using 2.0 μg of 20920-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of HEK-293 using 20920-1-AP

  IP result of anti-ITCH (IP:20920-1-AP, 4ug; Detection:20920-1-AP 1:300) with HEK-293 cells lysate 2400 ug.

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  IHC staining of human breast cancer using 20920-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20920-1-AP (ITCH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20920-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20920-1-AP (ITCH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562, cellules NIH/3T3, cellules Raji, cellules U-251
• Résultats positifs en IP: cellules HEK-293, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below

Informations sur le produit

20920-1-AP cible ITCH dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ITCH Protéine recombinante Ag15120
• Nom complet: itchy E3 ubiquitin protein ligase homolog (mouse)
• Masse moléculaire calculée: 903 aa, 103 kDa
• Poids moléculaire observé: 103 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC011571
• Symbole du gène: ITCH
• Identification du gène (NCBI): 83737
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ITCH is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. It is involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP. ITCH can be cleaved by caspase-3, -6, and -7 on residue Asp240 and form a 65 kDa cleaved form (PMID: 19541840).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Heat stress activates YAP/TAZ to induce the heat shock transcriptome.; Authors - Min Luo ; KD Validated
human	WB	Dev Cell; ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.; Authors - Jie Ran ; KD Validated
human	WB	Theranostics; ACSS3 represses prostate cancer progression through downregulating lipid droplet-associated protein PLIN3.; Authors - Lijie Zhou
human	WB,IP	Clin Transl Med; GRAMD4 inhibits tumour metastasis by recruiting the E3 ligase ITCH to target TAK1 for degradation in hepatocellular carcinoma.; Authors - Qian Yun Ge ; KD Validated
human	WB, CoIP	Front Oncol; Coiled-Coil Domain-Containing 68 Downregulation Promotes Colorectal Cancer Cell Growth by Inhibiting ITCH-Mediated CDK4 Degradation.; Authors - Cong Wang ; KD Validated
human	WB	J Mol Med (Berl); Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology.; Authors - Yuhong Zhang