Anticorps Polyclonal de lapin anti-Islet 1

Islet 1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 15661-1-AP

Synonymes

ISL1, Islet-1, ISLET1, ISL LIM homeobox 1, Isl 1

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Galerie de données de validation

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  WB analysis of BxPC-3 using 15661-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15661-1-AP (ISL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15661-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15661-1-AP (ISL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15661-1-AP

  IP result of anti-Islet 1 (IP:15661-1-AP, 4ug; Detection:15661-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human pancreas using 15661-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15661-1-AP (Islet 1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 15661-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15661-1-AP (Islet 1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas using 15661-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15661-1-AP (Islet 1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas cancer using 15661-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15661-1-AP (Islet 1 Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of SH-SY5Y using 15661-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Islet 1 antibody (15661-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 15661-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human Islet 1 (15661-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,
• Résultats positifs en FC (Intra): cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

15661-1-AP cible Islet 1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Islet 1 Protéine recombinante Ag8088
• Nom complet: ISL LIM homeobox 1
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39-45 kDa
• Numéro d’acquisition GenBank: BC031213
• Symbole du gène: Islet 1
• Identification du gène (NCBI): 3670
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts.

Publications

Species	Application	Title
human	IF	Front Neurol; Tranylcypromine Causes Neurotoxicity and Represses BHC110/LSD1 in Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoids Model.; Authors - Jing Huang
human	IF	Neuroscience; PMID: 30599272; Authors - Fangkun Liu
human		ACS Chem Neurosci; Amyotrophic Lateral Sclerosis-Associated Persistent Organic Pollutant cis-Chlordane Causes GABAA-Independent Toxicity to Motor Neurons, Providing Evidence toward an Environmental Component of Sporadic Amyotrophic Lateral Sclerosis; Authors - Daniel Kulick

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (06-10-2019)

ISL1 antibody recognizes a couple bands above 50kD which is a bit off from the expected molecular weight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

Islet 1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:15661-1-AP)