Anticorps Polyclonal de lapin anti-IRF8

IRF8 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 24437-1-AP

Synonymes

H ICSBP, ICSBP, ICSBP1, IRF 8, IRF8

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Galerie de données de validation

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  WB analysis of Raji using 24437-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24437-1-AP (IRF8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

24437-1-AP cible IRF8 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IRF8 Protéine recombinante Ag19909
• Nom complet: ICSBP1
• Masse moléculaire calculée: 426 aa, 48 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC126247
• Symbole du gène: IRF8
• Identification du gène (NCBI): 3394
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IRFs comprise a family of transcription factors that function within the Jak/Stat pathway to regulate IFN and IFN-inducible gene expression in response to viral infection. IRFs predominantly express in lymphoid tissues and play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E). IRF-8/ICSCP is expressed predominately in hematopoietic cells and is further increased upon treatment with IFN (2111015,1460054). IRF-8 can function as a transcription repressor of ICS-containing promoters (1460054). Expression of IRF-8 can lead to the down-regulation of the anti-apoptotic protein Bcl-2 (14656881). Originally described as being induced by IFN-γ, IRF-8 expression is also elevated by IRF-α as well as IL-12 in NK and T cells (14581002). IRF-8 deficient mice have enhanced susceptibility to various pathogens and impaired production of IFNs, as well as deregulated hematopoiesis that resembles chronic myelogenous leukemia (9120398). IRF-8 also regulates bone metabolism by suppressing osteoclast formation (19718038).This antibody specifically recognizes the 48kd IRF8 protein.