Anticorps Polyclonal de lapin anti-MUM1/IRF4

• Phare
• Validé par KD/KO

MUM1/IRF4 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (1)

N° de cat : 11247-2-AP

Synonymes

IRF4, Lymphocyte-specific interferon regulatory factor, LSIRF, IRF-4, IRF 4

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Galerie de données de validation

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  WB analysis using 11247-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 11247-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11247-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 11247-2-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11247-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 11247-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 11247-2-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 11247-2-AP

  IP result of anti-MUM1/IRF4 (IP:11247-2-AP, 3ug; Detection:11247-2-AP 1:500) with mouse spleen tissue lysate 4000ug.

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  IHC staining of human thymus using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thymus using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 11247-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of Ramos using 11247-2-AP

  1X10^6 Ramos cells were intracellularly stained with 0.5 ug Anti-Human MUM1/IRF4 (11247-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of Ramos using 11247-2-AP

  (blue)1x10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human MUM1/IRF4 (11247-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) at dilution 1:1000(red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules Jurkat, cellules Raji, RAW264.7, tissu de thymus de souris, tissu pulmonaire de souris, tissu splénique de rat
• Résultats positifs en IP: tissu splénique de souris
• Résultats positifs en IHC: tissu de thymus humain, tissu d'amygdalite humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules Ramos,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 5 publications below

Informations sur le produit

11247-2-AP cible MUM1/IRF4 dans les applications de WB, IHC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MUM1/IRF4 Protéine recombinante Ag1762
• Nom complet: IRF 4
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC015752
• Symbole du gène: IRF4
• Identification du gène (NCBI): 3662
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

Publications

Species	Application	Title
	WB	Cancer Commun (Lond); Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies; Authors - Lu Tang
human,mouse	WB	Diabetes; Obese Skeletal Muscle-Expressed Interferon Regulatory Factor 4 Transcriptionally Regulates Mitochondrial Branched-Chain Aminotransferase Reprogramming Metabolome.; Authors - Ting Yao ; KD Validated
mouse	WB	Nanotoxicology; Macrophage polarization and activation at the interface of multi-walled carbon nanotube-induced pulmonary inflammation and fibrosis.; Authors - Jie Dong
human	IHC	Diabetes Res Clin Pract; fMaggot treatment promotes healing of diabetic foot ulcer wounds possibly by upregulating Treg levels.; Authors - Jie Zhang
mouse	IHC	Int Immunopharmacol; IRF-4 deficiency reduces inflammation and kidney fibrosis after folic acid-induced acute kidney injury.; Authors - Meixin Chen ; KO Validated
mouse	WB,IHC	Mediators Inflamm; MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression.; Authors - Aijun Jia

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shunbin (Verified Customer) (05-01-2023)

One of the best antibody we have used for western blotting. 1:10,000 produces a single specific band. original concentration: 440 ug/ml

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: B/T cell lines

MUM1/IRF4 Antibody Western Blot validation (1:10,000 dilution) in B/T cell lines (Cat no:11247-2-AP)