Anticorps Polyclonal de lapin anti-IREB2

• Phare
• Validé par KD/KO

IREB2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 23829-1-AP

Synonymes

ACO3, IRE BP 2, IREB2, Iron regulatory protein 2, IRP2, IRP2AD

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Galerie de données de validation

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  WB analysis of mouse lung using 23829-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 23829-1-AP (IREB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 23829-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23829-1-AP (IREB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-13 using 23829-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 23829-1-AP (IREB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 23829-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 23829-1-AP (IREB2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 23829-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 23829-1-AP (IREB2 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 23829-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23829-1-AP (IREB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 23829-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23829-1-AP (IREB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 23829-1-AP

  Immunofluorescent analysis of A549 cells using 23829-1-AP (IREB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules A549, cellules PC-13
• Résultats positifs en IHC: tissu splénique humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

23829-1-AP cible IREB2 dans les applications de WB, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IREB2 Protéine recombinante Ag20653
• Nom complet: iron-responsive element binding protein 2
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 90-105 kDa
• Numéro d’acquisition GenBank: BC017880
• Symbole du gène: IREB2
• Identification du gène (NCBI): 3658
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IREB2 is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner.

Publications

Species	Application	Title
mouse	WB	EMBO Rep; The deubiquitinase OTUD1 enhances iron transport and potentiates host antitumor immunity.; Authors - Jia Song
human	WB	Cell Mol Life Sci; Phenazine derivatives attenuate the stemness of breast cancer cells through triggering ferroptosis.; Authors - Yue Yang
human	WB,IF,CoIP	Free Radic Biol Med; Iron regulatory protein 2 is required for artemether -mediated anti-hepatic fibrosis through ferroptosis pathway.; Authors - Yujia Li ; KD Validated
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis.; Authors - Haipeng Cheng
human	WB	Nutrients; Iron Transport from Ferrous Bisglycinate and Ferrous Sulfate in DMT1-Knockout Human Intestinal Caco-2 Cells.; Authors - Xiaonan Yu
mouse	WB,IF	Neurochem Int; Activation of vitamin D receptor inhibits Tau phosphorylation is associated with reduction of iron accumulation in APP/PS1 transgenic mice.; Authors - Ting-Yao Wu