Anticorps Polyclonal de lapin anti-INTS9

INTS9 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11657-1-AP

Synonymes

CPSF2L, INT9, integrator complex subunit 9, INTS9, RC 74, RC74

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Galerie de données de validation

• 

  WB analysis of Jurkat using 11657-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11657-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11657-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11657-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11657-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11657-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11657-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11657-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11657-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11657-1-AP

  IP result of anti-INTS9 (IP:11657-1-AP, 4ug; Detection:11657-1-AP 1:1000) with HeLa cells lysate 880ug.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IP: See 2 publications below

Informations sur le produit

11657-1-AP cible INTS9 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: INTS9 Protéine recombinante Ag2226
• Nom complet: integrator complex subunit 9
• Masse moléculaire calculée: 658 aa, 74 kDa
• Poids moléculaire observé: 74 kDa
• Numéro d’acquisition GenBank: BC025267
• Symbole du gène: INTS9
• Identification du gène (NCBI): 55756
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module.; Authors - Kevin Sabath
human	WB	Protein Expr Purif; CRISPR-Cas9 mediated genetic engineering for the purification of the endogenous integrator complex from mammalian cells.; Authors - David Baillat
human	WB,IP	Mol Cell; Integrator facilitates RNAPII removal to prevent transcription-replication collisions and genome instability; Authors - Rahul Bhowmick
human	WB,IP	Mol Cell; Cytoplasmic binding partners of the Integrator endonuclease INTS11 and its paralog CPSF73 are required for their nuclear function; Authors - Min-Han Lin