Anticorps Polyclonal de lapin anti-INTS5

INTS5 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 14069-1-AP

Synonymes

INT5, integrator complex subunit 5, INTS5, KIAA1698

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Galerie de données de validation

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  WB analysis using 14069-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14069-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14069-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14069-1-AP (INTS5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14069-1-AP

  IP result of anti-INTS5 (IP:14069-1-AP, 4ug; Detection:14069-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human prostate cancer using 14069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14069-1-AP (INTS5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14069-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14069-1-AP (INTS5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14069-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14069-1-AP (INTS5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules NIH/3T3, cellules SKOV-3, tissu cérébral humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

14069-1-AP cible INTS5 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: INTS5 Protéine recombinante Ag5129
• Nom complet: integrator complex subunit 5
• Masse moléculaire calculée: 108 kDa
• Poids moléculaire observé: 108 kDa
• Numéro d’acquisition GenBank: BC060841
• Symbole du gène: INTS5
• Identification du gène (NCBI): 80789
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

INTS5, also named as INT5 and KIAA1698, is a component of the Integrator complex which involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

Publications

Species	Application	Title
mouse	WB	Science; Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.; Authors - Hai Zheng
human	WB	Nat Commun; INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex; Authors - Lauren G. Mascibroda
human	WB	Cell Rep; A combinatorial approach to uncover an additional Integrator subunit; Authors - Sarah R Offley
human	WB	Cell Rep; RPAP2 regulates a transcription initiation checkpoint by inhibiting assembly of pre-initiation complex.; Authors - Xinxin Wang
human	WB	Mol Cell; INTAC endonuclease and phosphatase modules differentially regulate transcription by RNA polymerase II; Authors - Shibin Hu