Anticorps Polyclonal de lapin anti-INSIG2

• Phare
• Validé par KD/KO

INSIG2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 24766-1-AP

Synonymes

INSIG 2, INSIG2

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Galerie de données de validation

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  WB analysis of rat liver using 24766-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 24766-1-AP (INSIG2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 24766-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 24766-1-AP (INSIG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 24766-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24766-1-AP (INSIG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 24766-1-AP

  IP result of anti-INSIG2 (IP:24766-1-AP, 4ug; Detection:24766-1-AP 1:500) with mouse liver tissue lysate 3600ug.

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  IHC staining of human liver using 24766-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24766-1-AP (INSIG2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 24766-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24766-1-AP (INSIG2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 24766-1-AP

  Immunofluorescent analysis of HepG2 cells using 24766-1-AP (INSIG2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 24766-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using INSIG2 antibody (24766-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu hépatique de rat, cellules A549, cellules SGC-7901
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

24766-1-AP cible INSIG2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: INSIG2 Protéine recombinante Ag14072
• Nom complet: INSIG 2
• Masse moléculaire calculée: 225 aa, 25 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC022475
• Symbole du gène: INSIG2
• Identification du gène (NCBI): 51141
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

INSIG2 mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. INSIG2 may bring HMGCR into ubiquitin-mediated proteasomal degradation. Insig2 protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. INSIG-2 may play an important role in therapy of hypercholesterolemia.

Publications

Species	Application	Title
human	WB,IP	Nature; The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.; Authors - Daqian Xu ; KD Validated
human	WB,IF	Mol Metab; CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.; Authors - Han Zeng ; KD Validated
mouse	WB,IP	Int J Biol Sci; The GR-gp78 Pathway is involved in Hepatic Lipid Accumulation Induced by Overexpression of 11β-HSD1.; Authors - Mengliang Hu
human	WB	Am J Cancer Res; p38α/S1P/SREBP2 activation by the SAM-competitive EZH2 inhibitor GSK343 limits its anticancer activity but creates a druggable vulnerability in hepatocellular carcinoma.; Authors - Pei-Ming Yang
human	WB	Proteomes; A Multi-Level Systems Biology Analysis of Aldrin's Metabolic Effects on Prostate Cancer Cells; Authors - Carmen Bedia ; KD Validated
mouse	WB	Nat Commun; In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion; Authors - Hasan Vatandaslar