Anticorps Polyclonal de lapin anti-INSIG1

• Phare
• Validé par KD/KO

INSIG1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 22115-1-AP

Synonymes

CL 6, INSIG 1, INSIG1, insulin induced gene 1, Insulin induced gene 1 protein

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Galerie de données de validation

• 

  WB analysis of A549 using 22115-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 22115-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 22115-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 22115-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22115-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HepG2, cellules MCF-7, cellules SGC-7901
• Résultats positifs en IHC: tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

22115-1-AP cible INSIG1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: INSIG1 Protéine recombinante Ag17420
• Nom complet: insulin induced gene 1
• Masse moléculaire calculée: 277 aa, 30 kDa
• Poids moléculaire observé: 28-31 kDa, 66-70 kDa
• Numéro d’acquisition GenBank: BC001880
• Symbole du gène: INSIG1
• Identification du gène (NCBI): 3638
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Insulin-induced genes (INSIGs), which consist of two isoforms (INSIG1 and INSIG2), are representative endoplasmic reticulum (ER)-resident oxysterol-binding proteins (OSBPs). INSIG1 is part of negative regulatory feedback, acting as a brake on SREBP transcriptional function (PMID: 23919961). INSIG1 also has a secondary function mediating sterolaccelerated proteolytic degradation of HMG CoA reductase, thus impacting the mevalonate pathway. Suppression of INSIG1 function promotes hepatic lipid remodelling and restrains NASH (non-alcoholic steatohepatitis) progression(PMID: 33722690).

Publications

Species	Application	Title
human	WB,IF	Nature; The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.; Authors - Daqian Xu ; KD Validated
	WB	Transl Psychiatry; A potential mechanism underlying atypical antipsychotics-induced lipid disturbances.; Authors - H L Cai
	WB	Eur Neuropsychopharmacol; Vitamin D deficiency exacerbates atypical antipsychotic-induced metabolic side effects in rats: Involvement of the INSIG/SREBP pathway.; Authors - Ruili Dang
	WB	Mol Nutr Food Res; (-)-Epicatechin regulates blood lipids and attenuates hepatic steatosis in rats fed high-fat diet(†).; Authors - Hui Cheng
	WB,IHC	J Physiol Biochem; Renal tubule ectopic lipid deposition in diabetic kidney disease rat model and in vitro mechanism of leptin intervention.; Authors - Shasha Liu
human	IF	JCI Insight; Hypomorphic ASGR1 modulates lipid homeostasis via INSIG1-mediated SREBP signaling suppression.; Authors - Yingying Xu