Anticorps Polyclonal de lapin anti-ING1

ING1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 17921-1-AP

Synonymes

ING1, Inhibitor of growth protein 1, p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a

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Galerie de données de validation

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  WB analysis of mouse liver using 17921-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17921-1-AP (ING1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17921-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17921-1-AP (ING1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 17921-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 17921-1-AP (ING1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules A375

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

17921-1-AP cible ING1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ING1 Protéine recombinante Ag12338
• Nom complet: inhibitor of growth family, member 1
• Masse moléculaire calculée: 279aa,32 kDa; 422aa,47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC093942
• Symbole du gène: ING1
• Identification du gène (NCBI): 3621
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ING1, also inhibitor of growth protein 1, is a 422 amino acid protein, which contains 1 PHD-type zinc finger and belongs to the ING family. ING1 localizes in the nucleus and interacts with TP53. It exsits as varous isoforms and isoform 1 is a 47 kDa protein. Isoform 2 is a 33 kDa protein and expressed in all normal tissues, as well as in all breast cancer and melanoma cell lines examined. ING1 cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. It is implicated as a tumor suppressor gene.