Anticorps Polyclonal de lapin anti-IMPDH2

• Phare
• Validé par KD/KO

IMPDH2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications

51

N° de cat : 12948-1-AP

Synonymes

IMP dehydrogenase 2, IMPD 2, IMPD2, IMPDH 2, IMPDH II, IMPDH2

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Galerie de données de validation

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  WB analysis of A549 using 12948-1-AP

  WB result of IMPDH2 antibody (12948-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMPDH2 transfected A549 cells.

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  WB analysis using 12948-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12948-1-AP (IMPDH2 antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 12948-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 12948-1-AP (IMPDH2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12948-1-AP

  IP result of anti-IMPDH2 (IP:12948-1-AP, 4ug; Detection:12948-1-AP 1:20000) with HeLa cells lysate 1320 ug.

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  IHC staining of human lung cancer using 12948-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12948-1-AP (IMPDH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12948-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12948-1-AP (IMPDH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12948-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IMPDH2 antibody (12948-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules K-562, cellules MCF-7, tissu cardiaque de rat
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 25 publications below
• IHC: See 5 publications below
• IF: See 30 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

12948-1-AP cible IMPDH2 dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IMPDH2 Protéine recombinante Ag3632
• Nom complet: IMP (inosine monophosphate) dehydrogenase 2
• Masse moléculaire calculée: 514 aa, 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC012840
• Symbole du gène: IMPDH2
• Identification du gène (NCBI): 3615
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270) IMPDH2 is highly enriched in RR(filamentous rods and rings). This antibody may have cross-reaction with IMPDH1 due to the high homology.

Publications

Species	Application	Title
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
mouse	IF	J Clin Invest; Hirschsprung-like disease is exacerbated by reduced de novo GMP synthesis.; Authors - Jonathan I Lake
human	WB	Mol Ther; IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.; Authors - Hailong Ruan ; KD Validated
human	IF	Autoimmun Rev; Anti-rods/rings autoantibodies in a patient with pancreatic injury.; Authors - Shingo Katayama
human	RIP	Cancer Lett; Circular RNA circPFKP promotes cell proliferation by activating IMPDH2 in prostate cancer.; Authors - Shiyu Wang
human	WB	Elife; SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome.; Authors - Michela Zaffagni