Anticorps Polyclonal de lapin anti-IL-24

IL-24 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12064-1-AP

Synonymes

C49A, FISP, IL 24, IL10B, IL24, IL-24, interleukin 24, mda 7, MDA7, Mob 5, ST16

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Galerie de données de validation

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  WB analysis of Recombinant protein using 12064-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 12064-1-AP (IL-24 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human malignant melanoma using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 10x lens)..

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  IHC staining of human malignant melanoma using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 40x lens)..

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  IHC staining of human lung cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12064-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12064-1-AP (IL-24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: Protéine recombinante
• Résultats positifs en IHC: tissu de mélanome malin humain, tissu de cancer de la prostate humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

12064-1-AP cible IL-24 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-24 Protéine recombinante Ag2701
• Nom complet: interleukin 24
• Masse moléculaire calculée: 207 aa, 24 kDa
• Numéro d’acquisition GenBank: BC009681
• Symbole du gène: IL-24
• Identification du gène (NCBI): 11009
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL24, also named as MDA-7, MDA7 or ST16, belongs to the IL-10 family. It has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. The induction of IL24 by oncogenes may support tumor growth at the early stages of cancer. The observed molecular weight of IL24 is reported between 25 and 35 kDa, a value higher than the deduced molecular weight of 23 kDa. This difference is likely due to post-translational modifications, such as glycosylation of the endogenously produced molecule.

Publications

Species	Application	Title
		Nat Med; Glycemic control in diabetes is restored by therapeutic manipulation of cytokines that regulate beta cell stress.; Authors - Sumaira Z Hasnain
human	WB	Front Oncol; Combination Therapy of Prostate Cancer by Oncolytic Adenovirus Harboring Interleukin 24 and Ionizing Radiation.; Authors - Li-Jun Mao
human	WB	J Exp Clin Cancer Res; Inhibition of autophagy by 3-MA enhances IL-24-induced apoptosis in human oral squamous cell carcinoma cells.; Authors - Jichen Li
human	WB	MedComm (2020); iPSC-derived NK cells with site-specific integration of CAR19 and IL24 at the multi-copy rDNA locus enhanced antitumor activity and proliferation; Authors - Yuxuan Zhang