Anticorps Monoclonal anti-IL-22RA2

IL-22RA2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

5G11A9

N° de cat : 66190-1-Ig

Synonymes

CRF2 10, CRF2 S1, CRF2X, IL 22 receptor subunit alpha 2, IL 22BP, IL 22R alpha 2, IL 22RA2, IL22BP, IL22RA2, IL-22RA2, Interleukin 22 binding protein, ZcytoR16

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Galerie de données de validation

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  WB analysis of Raji using 66190-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 66190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 66190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 66190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 66190-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-22RA2 antibody (66190-1-Ig, Clone: 5G11A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human breast cancer using 66190-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-22RA2 antibody (66190-1-Ig, Clone: 5G11A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Raji
• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du sein humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

66190-1-Ig cible IL-22RA2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-22RA2 Protéine recombinante Ag18682
• Nom complet: interleukin 22 receptor, alpha 2
• Masse moléculaire calculée: 263 aa, 31 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC125167
• Symbole du gène: IL22RA2
• Identification du gène (NCBI): 116379
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-22 receptor subunit alpha-2 (IL22RA2) is a soluble member of the type II cytokine receptor family. Through binding to IL22, IL22RA2 prevents the interaction of IL22 with the functional IL22-R complex and blocks the activity of IL22 (in vitro). IL22RA2 may play an important role as an IL22 antagonist in the regulation of inflammatory responses. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Publications

Species	Application	Title
pig	WB,IHC	Front Cell Infect Microbiol; Oral Administration of Lactobacillus rhamnosus GG Ameliorates Salmonella Infantis-Induced Inflammation in a Pig Model via Activation of the IL-22BP/IL-22/STAT3 Pathway.; Authors - Gui-Yan Yang
human	FC	Cells; The Rare IL22RA2 Signal Peptide Coding Variant rs28385692 Decreases Secretion of IL-22BP Isoform-1, -2 and -3 and Is Associated with Risk for Multiple Sclerosis.; Authors - Paloma Gómez-Fernández
human	WB,IF	Front Immunol; Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response.; Authors - Paloma Gómez-Fernández

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