Anticorps Polyclonal de lapin anti-IL-1RA

IL-1RA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 10844-1-AP

Synonymes

IL 1ra, IL1RN, IL-1RN, IL1RA, IL 1RN

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Galerie de données de validation

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  WB analysis using 10844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10844-1-AP (IL-1RA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10844-1-AP (IL-1RA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human oesophagus using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10844-1-AP (IL1RA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 10844-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IL-1RA antibody (10844-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A431 using 10844-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human IL-1RA (10844-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules MOLT-4, cellules RAW 264.7
• Résultats positifs en IHC: tissu œsophagien humain, tissu de cancer du côlon humain, tissu de côlon humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10844-1-AP cible IL-1RA dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-1RA Protéine recombinante Ag1277
• Nom complet: interleukin 1 receptor antagonist
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC009745
• Symbole du gène: IL-1RA
• Identification du gène (NCBI): 3557
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Clin Transl Oncol; Inhibitory effect and mechanism of mesenchymal stem cells on melanoma cells.; Authors - J Zhang
human	WB	NeuroImmune Pharm Ther; Resveratrol and its analogs suppress HIV replication, oxidative stress, and inflammation in macrophages; Authors - Santosh Kumar
human	IHC	Breast Cancer (Dove Med Press); Identification and Validation of Novel Metastasis-Related Immune Gene Signature in Breast Cancer; Authors - Shen Ma
mouse	IF	Front Immunol; The influence of angiopoietin-like protein 3 on macrophages polarization and its effect on the podocyte EMT in diabetic nephropathy; Authors - Yanli Ma