Anticorps Polyclonal de lapin anti-IL-15RA

IL-15RA Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 16744-1-AP

Synonymes

IL 15 receptor subunit alpha, IL 15R alpha, IL 15RA, IL15RA, IL-15RA, interleukin 15 receptor, alpha, sIL 15 receptor subunit alpha, sIL 15R alpha, sIL 15RA

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Galerie de données de validation

• 

  WB analysis of HeLa using 16744-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16744-1-AP (IL15RA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16744-1-AP (IL-15RA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16744-1-AP (IL-15RA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16744-1-AP (IL15RA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16744-1-AP (IL-15RA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16744-1-AP (IL-15RA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 16744-1-AP (IL-15RA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 16744-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 16744-1-AP (IL-15RA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

16744-1-AP cible IL-15RA dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-15RA Protéine recombinante Ag10151
• Nom complet: interleukin 15 receptor, alpha
• Masse moléculaire calculée: 231 aa, 24 kDa
• Poids moléculaire observé: 55-65 kDa
• Numéro d’acquisition GenBank: BC107777
• Symbole du gène: IL-15RA
• Identification du gène (NCBI): 3601
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897). The calculated molecular weight of full-length IL-15Rα is 28 kDa, IL-15Rα can be glycosylated, and larger apparent molecular weights of 55-65 kDa have been reported by some researches (PMID: 10480910; 15976182; 15671076; 17912462).

Publications

Species	Application	Title
human	IHC	Nat Commun; An Artificial Intelligence-guided signature reveals the shared host immune response in MIS-C and Kawasaki disease.; Authors - Pradipta Ghosh
human	WB	Clin Sci (Lond); HMGB1-induced autophagy facilitates hepatic stellate cells activation: a new pathway in liver fibrosis.; Authors - Jing Li
human	IHC	Stem Cells Dev; Human gastric cancer mesenchymal stem cell-derived IL15 contributes to tumor cell EMT via up-regulation of Tregs ratio and PD-1 expression in CD4+T cell.; Authors - Li Sun
human	IHC	Immunology; Interleukin-15 receptor α expression in inflammatory bowel disease patients before and after normalization of inflammation with infliximab.; Authors - Perrier Clémentine C
human,hamster	IHC	bioRxiv; AI-guided discovery of the invariant host response to viral pandemics.; Authors - Debashis Sahoo
human	IHC	EBioMedicine; AI-guided discovery of the invariant host response to viral pandemics; Authors - Debashis Sahoo