Anticorps Polyclonal de lapin anti-IL-10RA

IL-10RA Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 13356-1-AP

Synonymes

CD210, CDw210a, HIL 10R, IL 10 receptor subunit alpha, IL 10R subunit 1, IL 10R subunit alpha, IL 10R1, IL 10RA, IL-10 R alpha, IL10R, IL10RA, IL-10RA, interleukin 10 receptor, alpha

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Galerie de données de validation

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  WB analysis using 13356-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL-10RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13356-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL10RA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13356-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL10RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13356-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL10RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 13356-1-AP

  1X10^6 K-562 cells were stained with 0.2ug IL10RA antibody (13356-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique humain, tissu placentaire humain
• Résultats positifs en cytométrie: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

13356-1-AP cible IL-10RA dans les applications de WB, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-10RA Protéine recombinante Ag4156
• Nom complet: interleukin 10 receptor, alpha
• Masse moléculaire calculée: 578 aa, 63 kDa
• Poids moléculaire observé: 70-72 kDa, 90-95 kDa
• Numéro d’acquisition GenBank: BC028082
• Symbole du gène: IL10RA
• Identification du gène (NCBI): 3587
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays a critical role in maintaining immune system balance. IL-10 signals through a receptor complex consisting of two molecules of the IL-10R alpha-chain (IL-10RA) and two molecules of the accessory IL-10R beta-chain (IL-10RB) (PMID: 22236434). IL-10RA and IL-10RB are members of the type II cytokine receptor family. IL-10RA, also known as IL-10R1 or CD210, is expressed on most hematopoietic cells at a basal level but is upregulated by various cells upon activation (PMID: 24507158). IL-10RA serves as the ligand-binding subunit of the receptor complex. Upon binding of IL-10 to the IL-10R complex, the kinases JAK1 and TYK2 are activated and catalyze the phosphorylation of IL-10RA at specific intracellular tyrosine residues, leading to the recruitment and subsequent phosphorylation of STAT3 (PMID: 11244051; 10433356).

Publications

Species	Application	Title
mouse	IF	Front Immunol; The influence of angiopoietin-like protein 3 on macrophages polarization and its effect on the podocyte EMT in diabetic nephropathy; Authors - Yanli Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alessandra (Verified Customer) (11-29-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: fat