Anticorps Polyclonal de lapin anti-IL-36 Gamma

• Phare
• Validé par KD/KO

IL-36 Gamma Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 24723-1-AP

Synonymes

IL 1 epsilon, IL 1 related protein 2, IL 1F9, IL 1H1, IL 1RP2, IL1E, IL1F9, IL-1F9, IL1H1, IL1RP2, IL-36 gamma, IL-36γ, Interleukin 1 epsilon, Interleukin 1 family member 9, interleukin 1 family, member 9, Interleukin 1 homolog 1

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Galerie de données de validation

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  IHC staining of human colon cancer using 24723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24723-1-AP (IL36 gamma antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 24723-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24723-1-AP (IL36 gamma antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 24723-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24723-1-AP (IL36 gamma antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 24723-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24723-1-AP (IL36 gamma antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 24723-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24723-1-AP (IL36 gamma antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 24723-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24723-1-AP (IL36 gamma antibody at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de tumeur ovarienne humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

24723-1-AP cible IL-36 Gamma dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-36 Gamma Protéine recombinante Ag21489
• Nom complet: interleukin 1 family, member 9
• Masse moléculaire calculée: 169 aa, 19 kDa
• Numéro d’acquisition GenBank: BC098337
• Symbole du gène: IL-36 gamma/IL-1F9
• Identification du gène (NCBI): 56300
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL-36 Gamma is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection.

Publications

Species	Application	Title
human	IHC	J Cutan Pathol; IL-36α and IL-36γ expressions in the differential diagnosis of palmoplantar psoriasis and palmoplantar eczema: A retrospective histopathologic and immunohistochemical study.; Authors - Ozan Erdem
human	WB	Sci Immunol; Myeloid cell-derived proteases produce a proinflammatory form of IL-37 that signals via IL-36 receptor engagement; Authors - Graeme P Sullivan ; KD Validated