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Anticorps Monoclonal anti-NeutraKine® IL-3

NeutraKine® IL-3 Monoclonal Antibody for Neutralization, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

Neutralization, ELISA

Conjugaison

Non conjugué

CloneNo.

3C11G7

N° de cat : 69004-1-Ig

Synonymes

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, NeutraKine® IL-3, P cell stimulating factor

Galerie de données de validation

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  • Neutralization experiment of NeutraKine® IL-3 using NeutraKine® IL-3 Monoclonal antibody (69004-1-Ig)

    Neutralization experiment of NeutraKine® IL-3 using 69004-1-Ig

    Recombinant human IL-3 (Cat.NO. HZ-1074) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-3 (10 ng/mL HZ-1074) is neutralized by mouse anti-human IL-3 monoclonal antibody 69004-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 40-70 ng/mL.

  • Neutralization experiment of NeutraKine® IL-3 using NeutraKine® IL-3 Monoclonal antibody (69004-1-Ig)

    Neutralization experiment of NeutraKine® IL-3 using 69004-1-Ig

    Recombinant human IL-3 (Cat.NO. HZ-1074) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-3 (0.5 ng/mL HZ-1074) is neutralized by mouse anti-human IL-3 monoclonal antibody 69004-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.8-2.4 ng/mL.

Neutralization

Informations sur le produit

69004-1-Ig cible NeutraKine® IL-3 dans les applications de Neutralization, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène human Humankine IL-3 protein HZ-1074
Nom complet interleukin 3 (colony-stimulating factor, multiple)
Symbole du gène IL3
Identification du gène (NCBI) 3562
Conjugaison Non conjugué
Forme Lyophilized Powder
Méthode de purification Purification par protéine G
Tampon de stockage PBS stérile.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Informations générales

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders (PMID: 24103757;2698876;2809196).

This antibody can be used to neutralize the bioactivity of IL-3.