Anticorps Monoclonal anti-NeutraKine® IL-12/IL-23 p40

NeutraKine® IL-12/IL-23 p40 Monoclonal Antibody for Neutralization, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

Neutralization, ELISA

Conjugaison

Non conjugué

CloneNo.

2A9H6

N° de cat : 69006-1-Ig

Synonymes

IL-12/IL-23 p40, IL12B, 2A9H6, CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit

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Galerie de données de validation

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  Neutralization experiment of NeutraKine® IL-23 p40 using 69006-1-Ig

  Recombinant human IL-23 (Cat.NO. HZ-1254) induces invitro cultured mouse splenocytes to secret IL-17A in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-23 (4 ng/mL HZ-1254) is neutralized by mouse anti-human IL-23 p40 monoclonal antibody 69006-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 8-30 ng/mL.

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  Neutralization experiment of NeutraKine® IL-23 p40 using 69006-1-Ig

  Recombinant human IL-23 (Cat.NO. HZ-1254) induces in vitro cultured mouse splenocytes to secret IL-17A in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-23 (4 ng/mL HZ-1254) is neutralized by mouse anti-human IL-23 p40 monoclonal antibody 69006-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 8-30 ng/mL. The NeutraControl mouse anti-human IL-23 p40 monoclonal antibody 69506-1-Ig could recognize human IL-23, but could not neutralize human IL-23 in the range of 0.244-4000ng/mL(green curve, refer to top X-right Y axis).

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  ELISA experiment of Recombinant protein using 69006-1-Ig

  Indirect ELISA was carried out by coating recombinant Human IL-23 (Cat.NO. HZ-1254) at 70 ng/well followed by blocking and adding serial diluted IL-23 antibody 69006-1-Ig and 69506-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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  ELISA experiment of Recombinant protein using 69006-1-Ig

  Indirect ELISA was carried out by coating recombinant Human IL-12 (Cat.NO. HZ-1256) and IL-12 p40 (Cat.NO. HZ-1321) respectively at 70 ng/well followed by blocking and adding serial diluted 69029-1-Ig, 69529-1-Ig and 69006-1-Ig respectively. HRP-goat anti-mouse was used for detection. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. The result suggests that 69029-1-Ig and 69529-1-Ig only recognize IL-12(p35 and p40 heterodimer) but not recognize IL-12/IL-23 p40.

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Neutralization

Informations sur le produit

69006-1-Ig cible NeutraKine® IL-12/IL-23 p40 dans les applications de Neutralization, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: human Humankine IL-12/IL-23 p40 protein HZ-1254
• Nom complet: interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
• Masse moléculaire calculée: 328 aa, 37 kDa
• Numéro d’acquisition GenBank: BC067498
• Symbole du gène: IL12B
• Identification du gène (NCBI): 51561,3593
• Conjugaison: Non conjugué
• Forme: Lyophilized Powder
• Méthode de purification: Purification par protéine G
• Tampon de stockage: Sterile PBS
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Informations générales

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

This antibody can be used to neutralize the bioactivity of IL-12/IL-23 p40.

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