Anticorps Monoclonal anti-IL-12/IL-23 p40

IL-12/IL-23 p40 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

C17.8

N° de cat : CL488-65097

Synonymes

Il 12b, Il 12p40, IL-12/IL-23 p40, Il12b, Il-12b, Il12p40, Il-12p40, interleukin 12b, p40

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Galerie de données de validation

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  FC experiment of Balb/c mouse peritoneal macrophages using CL488-65097

  IFN-γ primed Balb/c mouse peritoneal macrophages stimulated with LPS in the presence of a protein transport inhibitor were treated with fixation and permeabilization reagents and intracellularly stained with CoraLite® Plus 488-conjugated Rat IgG2a Isotype Control (left) or 5 ul CoraLite® Plus 488 Anti-Mouse IL-12/IL-23 p40 (CL488-65097, Clone: C17.8) (right).

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Applications testées

• Résultats positifs en cytométrie: macrophages péritonéaux de souris Balb/c

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65097 cible IL-12/IL-23 p40 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-12 p70 recombinante de souris exprimée par CHO
• Nom complet: interleukin 12b
• Numéro d’acquisition GenBank: BC103608
• Symbole du gène: Il12b
• Identification du gène (NCBI): 16160
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C