Anticorps Monoclonal anti-IL-1β

IL-1β Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2A7C6

N° de cat : CL750-65276

Synonymes

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

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Galerie de données de validation

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  FC experiment of human PBMCs using CL750-65276

  1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with CoraLite® Plus 555 Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite® Plus 750 Anti-Human IL-1β (CL750-65276, Clone:2A7C6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

• 

  FC experiment of human PBMCs using CL750-65276

  1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 750 Anti-Human IL-1β (CL750-65276, Clone:2A7C6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 750 Anti-Human IL-1β (CL750-65276, Clone:2A7C6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: LPS and Brefeldin A treated human PBMCs

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-65276 cible IL-1β dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-1β Protéine recombinante HZ-1164
• Nom complet: interleukin 1, beta
• Masse moléculaire calculée: 269 aa, 31 kDa
• Numéro d’acquisition GenBank: BC008678
• Symbole du gène: IL1B
• Identification du gène (NCBI): 3553
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β (IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. (PMID: 8630372; 12401481; 23704929; 24618930)