Anticorps Polyclonal de lapin anti-IKZF1

IKZF1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 12016-1-AP

Synonymes

DNA binding protein Ikaros, hIk 1, Hs.54452, IK1, IKAROS, IKZF1, LYF1, PRO0758, ZNFN1A1

---

Galerie de données de validation

• 

  WB analysis of HeLa using 12016-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12016-1-AP (IKZF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12016-1-AP

  IP result of anti-IKZF1 (IP:12016-1-AP, 4ug; Detection:12016-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of human lymphoma using 12016-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12016-1-AP (IKZF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12016-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12016-1-AP (IKZF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

12016-1-AP cible IKZF1 dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IKZF1 Protéine recombinante Ag2631
• Nom complet: IKAROS family zinc finger 1 (Ikaros)
• Masse moléculaire calculée: 477 aa, 53 kDa
• Poids moléculaire observé: 50-70 kDa
• Numéro d’acquisition GenBank: BC018349
• Symbole du gène: IKZF1
• Identification du gène (NCBI): 10320
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with 50-70 kDa.

Publications

Species	Application	Title
human	CoIP	Circ Res; A Novel Regulatory Mechanism of Smooth Muscle α-actin Expression by NRG-1/circACTA2/miR-548f-5p Axis.; Authors - Yan Sun
human	WB	Cancers (Basel); Mechanistic Basis for In Vivo Therapeutic Efficacy of CK2 Inhibitor CX-4945 in Acute Myeloid Leukemia.; Authors - Morgann Klink
human	WB,CoIP	Eur J Med Chem; Discovery of potential novel CRBN modulators by virtual screening and bioassay.; Authors - Feng Xiong
human	WB	Mol Oncol; Dual inhibition of DNMTs and EZH2 can overcome both intrinsic and acquired resistance of myeloma cells to IMiDs in a cereblon-independent manner.; Authors - Konstantinos Dimopoulos
human	IHC	Leuk Lymphoma; PMID: 29718735; Authors - Konstantinos Dimopoulos
mouse	WB	Eur J Med Chem; Discovery of novel BTK PROTACs with improved metabolic stability via linker rigidification strategy; Authors - Song Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Ryan (Verified Customer) (06-15-2018)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: zebrafish retina

IKZF1 Antibody Immunohistochemistry validation (1:100 dilution) in zebrafish retina (Cat no:12016-1-AP)