Anticorps Polyclonal de lapin anti-IKBKG

• Phare
• Validé par KD/KO

IKBKG Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

29

N° de cat : 18474-1-AP

Synonymes

IkB kinase-associated protein 1, I-kappa-B kinase subunit gamma, Fip3p, FIP-3, FIP3

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Galerie de données de validation

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  WB analysis using 18474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18474-1-AP (IKBKG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18474-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18474-1-AP (IKBKG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 18474-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18474-1-AP (IKBKG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 18474-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18474-1-AP (IKBKG antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse embryo using 18474-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 18474-1-AP (IKBKG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 18474-1-AP

  Immunofluorescent analysis of HeLa cells using 18474-1-AP (IKBKG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu embryonnaire de souris,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 28 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

18474-1-AP cible IKBKG dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IKBKG Protéine recombinante Ag13358
• Nom complet: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC012114
• Symbole du gène: IKBKG
• Identification du gène (NCBI): 8517
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. IKBKG is a predominant 48-kD protein and an N-terminally truncated protein of 45 kDa produced in smaller amounts and translated from methionine-38.

Publications

Species	Application	Title
mouse	WB	J Cell Biol; Caspase inhibition prolongs inflammation by promoting a signaling complex with activated RIPK1.; Authors - Xinyue Huang
human	WB	Cell Death Differ; IKK-β mediates hydrogen peroxide induced cell death through p85 S6K1.; Authors - Jia C-H CH ; KD Validated
human	WB	Mol Cell Proteomics; Quantification of Dynamic Protein Interactions and Phosphorylation in LPS Signaling Pathway by SWATH-MS; Authors - Xiurong Wu
human	WB	Mol Oncol; RIP1 modulates death receptor mediated apoptosis and autophagy in macrophages.; Authors - Zhenyu Yao
human	WB,IF	FASEB J; 3D human bone marrow stromal and endothelial cell spheres promote bone healing in an osteogenic niche.; Authors - Stefanie Inglis
human	WB	Cell Mol Immunol; PMID: 28392573; Authors - Yushun Wan

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