Anticorps Polyclonal de lapin anti-IKBKB

• Phare
• Validé par KD/KO

IKBKB Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

88

Avis (1)

N° de cat : 15649-1-AP

Synonymes

I kappa B kinase 2, I kappa B kinase beta, IKBKB, IKK B, IKK beta, IKK2, IKKB, IκB, NFKBIKB

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Galerie de données de validation

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  WB analysis of Jurkat using 15649-1-AP

  WB result of IKBKB antibody (15649-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-IKBKB transfected Jurkat cells.

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  WB analysis of Jurkat using 15649-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15649-1-AP (IKBKB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15649-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15649-1-AP (IKBKB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15649-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15649-1-AP (IKBKB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15649-1-AP

  IP result of anti-IKBKB (IP:15649-1-AP, 4ug; Detection:15649-1-AP 1:3000) with Jurkat cells lysate 1400 ug.

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  IHC staining of human liver cancer using 15649-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15649-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15649-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15649-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HepG2, cellules K-562
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 80 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

15649-1-AP cible IKBKB dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IKBKB Protéine recombinante Ag8191
• Nom complet: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• Masse moléculaire calculée: 756aa,81 kDa; 256aa,29 kDa
• Poids moléculaire observé: 80 kDa, 86 kDa, 87 and 29 kDa
• Numéro d’acquisition GenBank: BC006231
• Symbole du gène: IKBKB
• Identification du gène (NCBI): 3551
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IKBKB, also named as IKKB, IKK2, NFKBIKB and IKK-B, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and I-kappa-B kinase subfamily. IKBKB is a Serine kinase that plays an essential role in the NF-kappa-B signaling pathway. It acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. In addition to the NF-kappa-B inhibitors, IKBKB phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. It also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, IKBKB acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. This antibody can identify 4 isoform of IKBKB with the molecular weight of 80, 86, 87 and 29 kDa.

Publications

Species	Application	Title
mouse	WB	Drug Des Devel Ther; Sichen Formula Ameliorates Lipopolysaccharide-Induced Acute Lung Injury via Blocking the TLR4 Signaling Pathways; Authors - Li-Shan Yan
human	IF	Cell Death Discov; tRNA-derived fragment TRF365 regulates the metabolism of anterior cruciate ligament cells by targeting IKBKB.; Authors - Dianbo Long
rat	WB	Redox Biol; Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro.; Authors - Dejuan Li
		Br J Pharmacol; PMID: 28963856; Authors - Xiao Han
mouse	WB	J Agric Food Chem; A Flower-like Brain Targeted Selenium Nanocluster Lowers the Chlorogenic Acid Dose for Ameliorating Cognitive Impairment in APP/PS1 Mice; Authors - Zhiwei Li
human	WB	Aging (Albany NY); HSP90 acts as a senomorphic target in senescent retinal pigmental epithelial cells.; Authors - Dan-Dan Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Eugenia (Verified Customer) (03-16-2022)

Antibody worked well with specific band although a couple of non-specific bands also appeared.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: A549 lung cancer cell line