Anticorps Monoclonal anti-human IgM

human IgM Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

2D10B10

N° de cat : 66484-1-Ig

Synonymes

human IgM, IGHM, IgM, MU, VH

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Galerie de données de validation

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  WB analysis of Human plasma using 66484-1-Ig

  0.1 μL human plasma was subjected to SDS PAGE followed by western blot with 66484-1-Ig (IGHM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Human plasma using 66484-1-Ig

  0.1 μL human plasma was subjected to SDS PAGE followed by western blot with 66484-1-Ig (IGHM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66484-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66484-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66484-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:1000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66484-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66484-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66484-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66484-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66484-1-Ig (IGHM antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66484-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human appendicitis using 66484-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human appendicitis using 66484-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using human IgM antibody (66484-1-Ig, Clone: 2D10B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 66484-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66484-1-Ig (IGHM antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain, tissu d'appendicite humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:2000-1:6000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66484-1-Ig cible human IgM dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: human IgM Protéine recombinante Ag1459
• Nom complet: immunoglobulin heavy constant mu
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC009851
• Symbole du gène: IGHM
• Identification du gène (NCBI): 3507
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IGHM is the constant region of heavy chain of IgM. IgM is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The μ heavy chain disease is a rare lymphoproliferative disorder. Human immunoglobulin heavy chain locus translocations are associated with leukaemias and lymphomas, including multiple myeloma, mantle cell lymphoma, Burkitt's lymphoma and diffuse large B cell lymphoma. This antibody detects the heavy chain of human IgM (~75 kDa).

Publications

Species	Application	Title
human	IHC	J Inflamm Res; The m6A/m1A/m5C-Related Methylation Modification Patterns and Immune Landscapes in Rheumatoid Arthritis and Osteoarthritis Revealed by Microarray and Single-Cell Transcriptome; Authors - Haishi Zheng
human	WB	Sci Rep; Natural antibodies and CRP drive anaphylatoxin production by urate crystals.; Authors - Anne Kathrin Wessig
human	IF	Adv Sci (Weinh); Geometric Constraints Regulate Energy Metabolism and Cellular Contractility in Vascular Smooth Muscle Cells by Coordinating Mitochondrial DNA Methylation; Authors - Han Liu