Anticorps Polyclonal de lapin anti-IGF1B-Specific

• Phare
• Validé par KD/KO

IGF1B-Specific Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

IHC, IF-P, Dot blot, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 20215-1-AP

Synonymes

IBP1, IGF I, IGF1, IGF-1, IGF1A, IGF1B, IGF1B-Specific, IGFI, Insulin like growth factor I, Mechano growth factor, MGF, Somatomedin C

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Galerie de données de validation

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  IHC staining of human liver cancer using 20215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 20215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 20215-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 20215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 20215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20215-1-AP (IGF1B-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 20215-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using IGF1B-Specific antibody (20215-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du poumon humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique de souris,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

20215-1-AP cible IGF1B-Specific dans les applications de IHC, IF-P, Dot blot, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: insulin-like growth factor 1 (somatomedin C)
• Masse moléculaire calculée: 22 kDa
• Numéro d’acquisition GenBank: NM_001111285
• Symbole du gène: IGF1
• Identification du gène (NCBI): 3479
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IGF1B, also named as IBP1, MGF, IGF-I and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but have a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. The antibody is specific to isoform IGF-1B.

Publications

Species	Application	Title
mouse	Dot blot	Environ Health Perspect; Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice.; Authors - Jacob Raber
chicken		Oxid Med Cell Longev; IGF1 Knockdown Hinders Myocardial Development through Energy Metabolism Dysfunction Caused by ROS-Dependent FOXO Activation in the Chicken Heart.; Authors - Yafan Gong
rat		Cell Death Dis; miR-129 controls axonal regeneration via regulating insulin-like growth factor-1 in peripheral nerve injury.; Authors - Hui Zhu ; KD Validated
human		Front Bioeng Biotechnol; Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.; Authors - Kaile Zhang
		Chem Biol Interact; Oenothein B induced human non-small cell lung cancer A549 cell death by ROS-mediated PI3K/Akt/NF-κB signaling pathway.; Authors - Xiaodong Pei
		Mol Cell Biol; Histone Citrullination Represses MicroRNA Expression, Resulting in Increased Oncogene mRNAs in Somatolactotrope Cells.; Authors - Stanley B DeVore