Anticorps Polyclonal de lapin anti-IFT52

• Phare
• Validé par KD/KO

IFT52 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris et plus (1)

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (1)

N° de cat : 17534-1-AP

Synonymes

C20orf9, CGI 53, IFT52, NGD5, Protein NGD5 homolog

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Galerie de données de validation

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  WB analysis using 17534-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 17534-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17534-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MDCK using 17534-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 17534-1-AP

  IP result of anti-IFT52 (IP:17534-1-AP, 4ug; Detection:17534-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IP experiment of HeLa cells using 17534-1-AP

  IP result of anti-IFT52 (17534-1-AP for IP and Detection) with HeLa cell lysate.

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  IF Staining of HeLa using 17534-1-AP

  Immunofluorescent analysis of Hela cells, using IFT52 antibody 17534-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules LNCaP, cellules MDCK, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: tissu testiculaire de souris, cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

17534-1-AP cible IFT52 dans les applications de WB, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFT52 Protéine recombinante Ag11586
• Nom complet: intraflagellar transport 52 homolog (Chlamydomonas)
• Masse moléculaire calculée: 437 aa, 50 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC039831
• Symbole du gène: IFT52
• Identification du gène (NCBI): 51098
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 45-54kd.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; The cilia protein IFT88 is required for spindle orientation in mitosis.; Authors - Delaval Benedicte B
human	WB	Nat Commun; IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.; Authors - Nicolas Taulet
human	WB	Am J Hum Genet; Bi-allelic Mutations in TTC29 Cause Male Subfertility with Asthenoteratospermia in Humans and Mice.; Authors - Chunyu Liu
mouse	CoIP	Am J Hum Genet; Bi-allelic Mutations in TTC21A Induce Asthenoteratospermia in Humans and Mice.; Authors - Wangjie Liu
mouse	WB	EMBO J; Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.; Authors - Shichao Duan
Chicken	IP	Proc Natl Acad Sci U S A; FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.; Authors - Akira Honda

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Charlotte (Verified Customer) (07-22-2024)

I tested the antibody following the classic iF protocol: PFA 4% 10 min cold methanol 5 min 1% BSA in PBST 1/200 in blocking solution, O.N. at 4°C 5*5min PBST 1/500 in blocking solution, 1h at RT° 4*5min PBST + 5min PBS However, the result is very doty which makes it hard to interpret and to distinguish IFT52 real signal (signal should be similar to the ARL13B one or as dots along a line if not showing a continuous line). It could be due to the protocol that needs to be adjusted for this antibody or maybe the antibody works better in other cell lines

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: LIGHT2

IFT52 Antibody Immunofluorescence validation (1/200 dilution) in LIGHT2 (Cat no:17534-1-AP)