Anticorps Recombinant de lapin anti-IFN-gamma

IFN-gamma Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

6K20

N° de cat : 98001-1-RR

Synonymes

IFN Gamma, Ifng, interferon gamma, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFNG2

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Galerie de données de validation

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  FC experiment of wistar rat splenocytes using 98001-1-RR

  1X10^6 unstimulated or ConA, PMA and ionomycin stimulated (in the presence of protein transport inhibitors) Wistar rat splenocytes were intracellularly stained with 0.25 ug Anti-Rat IFN-gamma (98001-1-RR, Clone: 6K20) and PE-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody. Cells were co-stained with 0.25 ug APC Anti-Rat CD4 (OX-35) (APC-65179, Clone: OX-35). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98001-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98001-1-RR against Rat IFN-gamma were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en FC (Intra): ConA, PMA and ionomycin stimulated Wistar rat splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98001-1-RR cible IFN-gamma dans les applications de FC (Intra) et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interferon gamma
• Masse moléculaire calculée: 18 kDa
• Numéro d’acquisition GenBank: NM_138880
• Symbole du gène: IFN-gamma
• Identification du gène (NCBI): 25712
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Interferon-gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)

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