Anticorps Polyclonal de lapin anti-IFFO1

IFFO1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (2)

N° de cat : 16041-1-AP

Synonymes

HOM TES 103, IFFO, IFFO1, Tumor antigen HOM TES 103

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Galerie de données de validation

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  WB analysis of mouse brain using 16041-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16041-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16041-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16041-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16041-1-AP (IFFO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 16041-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16041-1-AP (IFFO1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 16041-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16041-1-AP (IFFO1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 16041-1-AP

  Immunofluorescent analysis of Hela cells, using IFFO1 antibody 16041-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral humain, tissu testiculaire de souris
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

16041-1-AP cible IFFO1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFFO1 Protéine recombinante Ag8915
• Nom complet: intermediate filament family orphan 1
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 75-85 kDa
• Numéro d’acquisition GenBank: BC004384
• Symbole du gène: IFFO1
• Identification du gène (NCBI): 25900
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFFO1, also named as IFFO and Tumor antigen HOM-TES-103, is a member of the intermediate filament family which consists of primordial components of the cytoskeleton and nuclear envelope that are mainly distributed in the nucleus, followed by the cytoskeleton and cytoplasm. IFFO1 in the nucleus was identified to combine with both XRCC4 and the nuclear lamina protein lamin A/C to be involved in nonhomologous DNA end joining (NHEJ). The expression of IFFO1 is associated with tumor progression. IFFO1 could be used as a potential marker to differentiate malignant ascites cell-free tumor DNA (cftDNA) of ovarian cancer from benign controls.

Publications

Species	Application	Title
human	IHC	Cancer Lett; METTL3-mediated N6-methyladenosine modification and HDAC5/YY1 promote IFFO1 downregulation in tumor development and chemo-resistance; Authors - Ye Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shinford (Verified Customer) (10-26-2023)

It's essential to exercise caution while cutting the membrane as the bands appeared very close to the 70kDa line. In my case, I experienced the loss of some bands, possibly due to the cut being too close to the border.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Cancer cell lines

FH

Maria Jose (Verified Customer) (09-28-2020)

The antibody is very unspecific. There are many bands in the western blot. It recognizes GST with very high affinity.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human