Anticorps Monoclonal anti-Iduronate 2 sulfatase

Iduronate 2 sulfatase Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1A3F9

N° de cat : 66112-1-Ig

Synonymes

IDS, iduronate 2 sulfatase, Idursulfase

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Galerie de données de validation

• 

  WB analysis of human placenta using 66112-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66112-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66112-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66112-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66112-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 66112-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66112-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66112-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50.

• 

  IHC staining of human liver using 66112-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50.

• 

  IF Staining of HepG2 using 66112-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

• 

  IF Staining of HepG2 using 66112-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Iduronate 2 sulfatase antibody (66112-1-Ig, Clone: 1A3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: placenta humain, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66112-1-Ig cible Iduronate 2 sulfatase dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Iduronate 2 sulfatase Protéine recombinante Ag19095
• Nom complet: iduronate 2-sulfatase
• Masse moléculaire calculée: 312aa,35 kDa; 550aa,62 kDa
• Poids moléculaire observé: 76 kDa, 55 kDa
• Numéro d’acquisition GenBank: BC006170
• Symbole du gène: IDS
• Identification du gène (NCBI): 3423
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).