Anticorps Polyclonal de lapin anti-IDH3A

• Phare
• Validé par KD/KO

IDH3A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

17

Avis (1)

N° de cat : 15909-1-AP

Synonymes

NAD(+)-specific ICDH subunit alpha, Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, IDH3, EC:1.1.1.41

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 15909-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15909-1-AP (IDH3A antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis using 15909-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15909-1-AP (IDH3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15909-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15909-1-AP (IDH3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15909-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15909-1-AP (IDH3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15909-1-AP

  IP result of anti-IDH3A (IP:15909-1-AP, 4ug; Detection:15909-1-AP 1:1000) with HepG2 cells lysate 2400ug.

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  IHC staining of human brain using 15909-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15909-1-AP (IDH3A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 15909-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15909-1-AP (IDH3A antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 15909-1-AP

  Immunofluorescent analysis of HepG2 cells using 15909-1-AP (IDH3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15909-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IDH3A antibody (15909-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 15909-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human IDH3A (15909-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

15909-1-AP cible IDH3A dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IDH3A Protéine recombinante Ag8662
• Nom complet: isocitrate dehydrogenase 3 (NAD+) alpha
• Masse moléculaire calculée: 366 aa, 40 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC021967
• Symbole du gène: IDH3A
• Identification du gène (NCBI): 3419
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Nat Neurosci; A glycolytic shift in Schwann cells supports injured axons.; Authors - Elisabetta Babetto
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
mouse	WB	EMBO J; Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues.; Authors - Yingjiang Xu
human	IHC	J Invest Dermatol; Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.; Authors - Zhichao Gu
human	WB	Oncotarget; CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.; Authors - Zhichao Gu
rat	WB	Cell Physiol Biochem; Energy Metabolism in the Bone is Associated with Histomorphometric Changes in Rats with Hyperthyroidism.; Authors - Zhuoqing Hu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Svitlana (Verified Customer) (03-19-2019)

SDS-PAGE: 15 ug protein lysate/well, 4-12% Bis-Tris.Transfer: Immobilon-FL transfer membranes O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1h at room temperature.Secondary Ab: IRDye 800CW Goat anti-Rabbit 1 h at room temperature.Lines on WB1. BioRad Precision Plus Protein standard2. Homogenate of human cortex tissue3. Non-synaptosomal mitochondria from human cortex4. Homogenate of mice brain tissue5. Non-synaptosomal mitochondria from mice brains

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissues, human and mouse

IDH3A Antibody Western Blot, validation (1:1000 dilution) in Brain tissues, human and mouse (Cat no:15909-1-AP)