Anticorps Polyclonal de lapin anti-IDE

• Phare
• Validé par KD/KO

IDE Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 21728-1-AP

Synonymes

Abeta degrading protease, IDE, INSULYSIN

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Galerie de données de validation

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  WB analysis using 21728-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21728-1-AP

  WB result of IDE antibody (21728-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDE transfected HeLa cells.

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  WB analysis of HepG2 using 21728-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 21728-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 21728-1-AP

  IP result of anti-IDE (IP:21728-1-AP, 4ug; Detection:21728-1-AP 1:500) with HepG2 cells lysate 1600ug.

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  IHC staining of human kidney using 21728-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 21728-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 21728-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 21728-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules DU 145, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu rénal humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 1 publications below

Informations sur le produit

21728-1-AP cible IDE dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IDE Protéine recombinante Ag16392
• Nom complet: Abeta degrading protease
• Masse moléculaire calculée: 1019 aa, 118 kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: BC096336
• Symbole du gène: IDE
• Identification du gène (NCBI): 3416
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IDE (INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531). This antibody is specific to IDE.

Publications

Species	Application	Title
human	WB	Brain; MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.; Authors - Bing-Lin Zhu
human	WB	Carbohydr Polym; A pectin from fruits of Lycium barbarum L. decreases β-amyloid peptide production through modulating APP processing.; Authors - Lishuang Zhou
human	WB	Cell Physiol Biochem; Novel Serum Biomarkers Detected by Protein Array in Polycystic Ovary Syndrome with Low Progesterone Level.; Authors - Qin Zheng
human	WB	Int J Biol Macromol; A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin.; Authors - Hui Zeng
human	WB	J Biol Chem; Cell surface expression of the major amyloid-β peptide (Aβ)-degrading enzyme, neprilysin, depends on phosphorylation by mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) and dephosphorylation by protein phosphatase 1a.; Authors - Naomasa Kakiya
	WB	Neurochem Int; Activation of α7 nicotinic acetylcholine receptor alleviates Aβ1-42-induced neurotoxicity via downregulation of p38 and JNK MAPK signaling pathways.; Authors - Ke-Wei Chang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (12-15-2020)

Non-specific band at around 60kDa was stronger than band for IDE, although IDE band could still be made out.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293T

IDE Antibody Western Blot validation (1:500 dilution) in HEK293T (Cat no:21728-1-AP)