Anticorps Polyclonal de lapin anti-ID1

• Phare
• Validé par KD/KO

ID1 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 18475-1-AP

Synonymes

bHLHb24, ID, ID1, Inhibitor of DNA binding 1

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Galerie de données de validation

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  WB analysis of PC-3 using 18475-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18475-1-AP (ID1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 18475-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18475-1-AP (ID1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 18475-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 18475-1-AP (ID1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-3
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

18475-1-AP cible ID1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ID1 Protéine recombinante Ag13359
• Nom complet: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC000613
• Symbole du gène: ID1
• Identification du gène (NCBI): 3397
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ID (inhibitor of DNA binding) proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID1 proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID proteins contribute to cell growth, senescence, differentiation, and angiogenesis. Inhibitor of differentiation or DNA binding-1 (ID1) interacts with the transactivation domain of p65 (p65TAD) both in vitro and in vivo. The molecular weight of ID1 is about 20 kDa.

Publications

Species	Application	Title
human,mouse	WB,IF	Nat Commun; ID1 expressing macrophages support cancer cell stemness and limit CD8+ T cell infiltration in colorectal cancer; Authors - Shuang Shang
rat	WB	Basic Res Cardiol; The miR-182/Myadm axis regulates hypoxia-induced pulmonary hypertension by balancing the BMP- and TGF-β-signalling pathways in an SMC/EC-crosstalk-associated manner; Authors - Yongyi Bai
mouse	IF	EMBO J; Defining multistep cell fate decision pathways during pancreatic development at single-cell resolution.; Authors - Xin-Xin Yu
mouse	WB	Clin Transl Med; Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.; Authors - Xi Dong
human,mouse	WB	Environ Health Perspect; Exposure to ambient air fine particulate matter prevents VEGF-induced mobilization of endothelial progenitor cells from the bone marrow.; Authors - Haberzettl Petra P
human	WB	Acta Pharmacol Sin; Pantoprazole ameliorates liver fibrosis and suppresses hepatic stellate cell activation in bile duct ligation rats by promoting YAP degradation; Authors - Zhen-ning Lu