Anticorps Polyclonal de lapin anti-ICT1

ICT1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 10403-1-AP

Synonymes

MRPL58, Large ribosomal subunit protein mL62, EC:3.1.1.29, DS-1, DS1

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Galerie de données de validation

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  WB analysis of A2780 using 10403-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10403-1-AP (ICT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 10403-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10403-1-AP (ICT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of SKOV-3 using 10403-1-AP

  IP result of anti-ICT1 (IP:10403-1-AP, 4ug; Detection:10403-1-AP 1:300) with SKOV-3 cells lysate 2000ug.

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  IF Staining of HeLa using 10403-1-AP

  Immunofluorescent analysis of HeLa cells using 10403-1-AP (ICT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules SKOV-3
• Résultats positifs en IP: cellules SKOV-3
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

10403-1-AP cible ICT1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ICT1 Protéine recombinante Ag0647
• Nom complet: immature colon carcinoma transcript 1
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 21-24 kDa
• Numéro d’acquisition GenBank: BC015335
• Symbole du gène: ICT1
• Identification du gène (NCBI): 3396
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is immature colon carcinoma transcript 1 (ICT1, synonym: DS-1) whose mRNA and protein were more highly expressed in undifferentiated than differentiated cells. This antibody is a rabbit polyclonal antibody raised against a full-length human ICT1 protein.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	Cell Rep; The Human Mitochondrial DEAD-Box Protein DDX28 Resides in RNA Granules and Functions in Mitoribosome Assembly.; Authors - Ya-Ting Tu
human	WB	Nucleic Acids Res; MPV17L2 is required for ribosome assembly in mitochondria.; Authors - Ilaria Dalla Rosa
human	WB	PLoS Genet; RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.; Authors - Aurelio Reyes
human	WB	Mitochondrion; Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria.; Authors - Dujeancourt Laurent L
human	WB	Biochem J; The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome.; Authors - Agata Rozanska