Anticorps Polyclonal de lapin anti-ICAM2

• Phare
• Validé par KD/KO

ICAM2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10121-2-AP

Synonymes

CD102, ICAM 2, ICAM2

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Galerie de données de validation

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  WB analysis of Jurkat using 10121-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10121-2-AP (ICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10121-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10121-2-AP (ICAM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 10121-2-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10121-2-AP (ICAM2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10121-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10121-2-AP (ICAM2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10121-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10121-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 10121-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10121-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10121-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules RAW 264.7, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du poumon humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

10121-2-AP cible ICAM2 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ICAM2 Protéine recombinante Ag0165
• Nom complet: intercellular adhesion molecule 2
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 55-80 kDa
• Numéro d’acquisition GenBank: BC003097
• Symbole du gène: ICAM2
• Identification du gène (NCBI): 3384
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187. This antibody got 55-80 kDa in western blotting maybe due to glycosylation.

Publications

Species	Application	Title
mouse	WB	Front Cell Infect Microbiol; Brain Microvascular Endothelial Cell-Derived HMGB1 Facilitates Monocyte Adhesion and Transmigration to Promote JEV Neuroinvasion.; Authors - Song-Song Zou
human	WB,IHC	PLoS One; ICAM-2 expression mediates a membrane-actin link, confers a nonmetastatic phenotype and reflects favorable tumor stage or histology in neuroblastoma.; Authors - Yoon Karina Jin KJ ; KD Validated
human	IHC	J Ovarian Res; The presence of living endometrial cells in ovarian endometriotic cyst fluid may contribute to the recurrence of endometriosis after surgical excision of endometriomas; Authors - Xinxin Xu
human	IHC	Hum Pathol; Intercellular adhesion molecule 1/2 and E-selectin in plasma cell mastitis: immunohistochemical study of 35 cases.; Authors - Yu Dong
human	IF	Stem Cell Res Ther; MSCs promote the efferocytosis of large peritoneal macrophages to eliminate ferroptotic monocytes/macrophages in the injured endometria; Authors - Jiali Wang