Anticorps Monoclonal anti-Histone H3

Histone H3 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

canin, chien, Humain, Lapin, poisson-zèbre, porc, poulet, rat, souris, Hamster, wheat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

1A2A3

N° de cat : 68345-1-Ig

Synonymes

H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, Histone-H3



Applications testées

Résultats positifs en WBcellules LNCaP, cellules CHO, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MDCK, cellules NIH/3T3, tissu cérébral de poulet, poisson-zèbre
Résultats positifs en IPcellules HeLa,
Résultats positifs en IHCtissu testiculaire de souris,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IFcellules MCF-7, cellules A431, cellules HeLa
Résultats positifs en cytométriecellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 9 publications below

Informations sur le produit

68345-1-Ig cible Histone H3 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons canin, chien, Humain, Lapin, poisson-zèbre, porc, poulet, rat, souris, Hamster, wheat

Réactivité canin, chien, Humain, Lapin, poisson-zèbre, porc, poulet, rat, souris, Hamster, wheat
Réactivité citéerat, Humain, souris
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène Peptide
Nom complet histone cluster 1, H3a
Poids moléculaire observé15 kDa
Numéro d’acquisition GenBankBC066245
Symbole du gène HIST1H3A
Identification du gène (NCBI) 8350
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.

Protocole

Product Specific Protocols
WB protocol for Histone H3 antibody 68345-1-IgDownload protocol
IHC protocol for Histone H3 antibody 68345-1-IgDownload protocol
IF protocol for Histone H3 antibody 68345-1-IgDownload protocol
IP protocol for Histone H3 antibody 68345-1-IgDownload protocol
FC protocol for Histone H3 antibody 68345-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice

Authors - Boying Zhao
ratWB

Int J Mol Sci

Ursolic Acid Alleviates Neuroinflammation after Intracerebral Hemorrhage by Mediating Microglial Pyroptosis via the NF-κB/NLRP3/GSDMD Pathway

Authors - Pan Lei
humanWB

Curr Med Chem

Resveratrol Inhibits Hepatocellular Carcinoma Progression Through Regulating Exosome Secretion

Authors - Kun Tong
mouseWB

Brain Res

Neuronal pyroptosis mediated by STAT3 in early brain injury after subarachnoid hemorrhage

Authors - Shengjie Tang
humanWB

J Virol

HIV-1 Gag co-localizes with euchromatin histone marks at the nuclear periphery

Authors - Jordan Chang
humanWB

J Eur Acad Dermatol Venereol

Serine deficiency exacerbates psoriatic skin inflammation by regulating S-adenosyl methionine-dependent DNA methylation and NF-κB signalling activation in keratinocytes

Authors - Qinqin Meng