Anticorps Polyclonal de lapin anti-HTR3A

• Phare
• Validé par KD/KO

HTR3A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10443-1-AP

Synonymes

5 HT 3, 5 HT3 A, 5 HT3A, 5 HT3R, 5 hydroxytryptamine receptor 3, 5HT3R, HTR3, HTR3A, Serotonin receptor 3A

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Galerie de données de validation

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  WB analysis of mouse lung using 10443-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10443-1-AP (HTR3A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10443-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10443-1-AP (HTR3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 10443-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10443-1-AP (HTR3A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 10443-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10443-1-AP (HTR3A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10443-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10443-1-AP (HTR3A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10443-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10443-1-AP (HTR3A Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10443-1-AP cible HTR3A dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HTR3A Protéine recombinante Ag0289
• Nom complet: 5-hydroxytryptamine (serotonin) receptor 3A
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC002354
• Symbole du gène: HTR3A
• Identification du gène (NCBI): 3359
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HTR3A (5-hydroxytryptamine receptor 3A, 5-HT3A) belongs to the ligand-gated ion channel receptor superfamily. It is the subunit A of the type 3 receptor for 5-hydroxytryptamine (5-HT, serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. So far, five distinct 5-HT3 receptor subunits (A-E) have been identified. HTR3A can form functional homomers or heteromers with HTR3B or HTR3C or HTR3D or HTR3E. 5-HT3 receptors are capable of mediating fast excitatory neurotransmission in the CNS and peripheral nervous system (PNS). (PMID: 23038271; 17073663)

Publications

Species	Application	Title
human	WB	Pain; Sex differences in serotonin enhancement of capsaicin-evoked calcitonin gene-related peptide release from human dental pulp.; Authors - Loyd Dayna R DR
rat	WB, IHC	Front Pharmacol; Involvement of 5-Serotonin and Substance p Pathways in Dichroa Alkali Salt-Induced Acute Pica in Rats.; Authors - Lina Ma
rat	IF	Sci Rep; Oral Phyto-thymol ameliorates the stress induced IBS symptoms.; Authors - Selvaraj Subramaniyam
mouse	WB	Front Endocrinol (Lausanne); Electroacupuncture Attenuates Post-Inflammatory IBS-Associated Visceral and Somatic Hypersensitivity and Correlates With the Regulatory Mechanism of Epac1-Piezo2 Axis.; Authors - Jing Guo
mouse	WB	Biochem Biophys Res Commun; FSH signaling is involved in affective disorders.; Authors - Wen-Kai Bi
human	WB	Oncol Lett; Downregulation of 5-hydroxytryptamine receptor 3A expression exerts an anticancer activity against cell growth in colorectal carcinoma cells in vitro.; Authors - Jian Tang ; KD Validated