Anticorps Polyclonal de lapin anti-HTATSF1

• Phare
• Validé par KD/KO

HTATSF1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 20805-1-AP

Synonymes

HIV 1 Tat specific factor 1, HIV Tat specific factor 1, HTATSF1, TAT SF1

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Galerie de données de validation

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  WB analysis of HeLa using 20805-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20805-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20805-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20805-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20805-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 20805-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20805-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 20805-1-AP

  IP result of anti-HTATSF1 (IP:20805-1-AP, 4ug; Detection:20805-1-AP 1:2000) with HepG2 cells lysate 2400ug.

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  IHC staining of human breast cancer using 20805-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 20805-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 20805-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 20805-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 20805-1-AP

  Immunofluorescent analysis of Hela cells, using HTATSF1 antibody 20805-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 20805-1-AP

  Immunofluorescent analysis of Hela cells, using HTATSF1 antibody 20805-1-AP at 1:50 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

20805-1-AP cible HTATSF1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HTATSF1 Protéine recombinante Ag14744
• Nom complet: HIV-1 Tat specific factor 1
• Masse moléculaire calculée: 755 aa, 86 kDa
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: BC009896
• Symbole du gène: HTATSF1
• Identification du gène (NCBI): 27336
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.; Authors - Jiao Zhao ; KD Validated
		Nucleic Acids Res; Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.; Authors - Xiaodong Lu
human	WB,IHC,IP	Cell Death Discov; Metabolic protein phosphoglycerate kinase 1 confers lung cancer migration by directly binding HIV Tat specific factor 1.; Authors - Yu-Chan Chang ; KD Validated
human	WB	Cell Rep; ArreSTick motif controls β-arrestin-binding stability and extends phosphorylation-dependent β-arrestin interactions to non-receptor proteins; Authors - András Dávid Tóth