Anticorps Polyclonal de lapin anti-HSPH1

HSPH1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 13383-1-AP

Synonymes

Antigen NY CO 25, Heat shock 110 kDa protein, Heat shock protein 105 kDa, HSP105, HSP105A, HSP105B, HSP110, HSPH1, KIAA0201, NY CO 25

---

Galerie de données de validation

• 

  WB analysis of MCF-7 using 13383-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13383-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13383-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13383-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13383-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13383-1-AP (HSPH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 13383-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13383-1-AP (HSPH1 Antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HeLa, cellules Jurkat, cellules K-562, tissu cérébral humain
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 4 publications below

Informations sur le produit

13383-1-AP cible HSPH1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSPH1 Protéine recombinante Ag4224
• Nom complet: heat shock 105kDa/110kDa protein 1
• Masse moléculaire calculée: 858 aa, 97 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC037553
• Symbole du gène: HSPH1
• Identification du gène (NCBI): 10808
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSP105, also known as HSP110 or HSPH1, belongs to the heat shock protein (HSP) family. Human HSP105 is a high-molecular-weight chaperone protein expressed at constitutively low levels as a cytoplasmic α-isoform and as an inducible nuclear β-isoform on exposure to various forms of stress. HSP105 is constitutively overexpressed in several solid tumors, including melanoma, breast, thyroid, and gastroenteric cancers, and exerts antiapoptotic functions. Recently HSP105 has been identified as a novel candidate biomarker of lymphoma aggressiveness. This antibody recognizes both HSP105α and HSP105β isoforms. Western blot analysis using this antibody detected a major band around 100-110 kDa in Jurkat cells.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition; Authors - Shan Lu
mouse	WB	Mol Ther Nucleic Acids; Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction; Authors - Jie Pan
mouse	WB	Mol Reprod Dev; Identification of Hsf1 as a novel androgen receptor-regulated gene in mouse sertoli cells.; Authors - Lihua Yang
human	IF	bioRxiv; Endothelial Cells are Heterogeneous in Different Brain Regions and are Dramatically Altered in Alzheimer's Disease; Authors - Annie Bryant
human	IF	J Neurosci; Endothelial Cells Are Heterogeneous in Different Brain Regions and Are Dramatically Altered in Alzheimer's Disease; Authors - Annie Bryant
rat	IF	Dev Cell; Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination; Authors - Li Li