Anticorps Polyclonal de lapin anti-HSP27

• Phare
• Validé par KD/KO

HSP27 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

37

Avis (1)

N° de cat : 18284-1-AP

Synonymes

HSPB1, HSP28, Hsp25, HMN2B, Estrogen-regulated 24 kDa protein

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Galerie de données de validation

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  WB analysis using 18284-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18284-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18284-1-AP

  WB result of HSP27 antibody (18284-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP27 transfected HeLa cells.

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  WB analysis of mouse liver using 18284-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 18284-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 18284-1-AP (HSP27 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18284-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18284-1-AP (HSP27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18284-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 18284-1-AP (HSP27 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 18284-1-AP

  Immunofluorescent analysis of MCF-7 cells, using HSPB1 antibody 18284-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 18284-1-AP

  Immunofluorescent analysis of Hela cells, using HSPB1 antibody 18284-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of MCF-7 using 18284-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human HSP27 (18284-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules C6, cellules HeLa, cellules HepG2, cellules U2OS, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du foie humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules MCF-7
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 29 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

18284-1-AP cible HSP27 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSP27 Protéine recombinante Ag13161
• Nom complet: heat shock 27kDa protein 1
• Masse moléculaire calculée: 19-23 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC012768
• Symbole du gène: HSPB1
• Identification du gène (NCBI): 3315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPB1, also known as heat shock protein 27 (HSP27), belongs to the small heat shock protein family which is induced in response to environmental challenges or/and developmental transitions. It is also an anti-apoptotic protein that plays crucial roles in tumorigenesis and cell survival and is reported to be an independent prognosis marker for cancer. Recently HSPB1 has been found to be a valuable marker for melanoma. In addition to the predicted 27 kDa, an extra 50-55 kDa representing dimeric form of HSPB1 may also be observed (PMID: 21353161). It's notable that mouse HSP27 is also named HSP25 and has the predicted MW between 19-23 kDa.

Publications

Species	Application	Title
human	WB	Nature; Oxidative cyclization reagents reveal tryptophan cation-π interactions; Authors - Xiao Xie
human	IF	Allergy; ACE2 downregulation in olfactory mucosa: Eosinophilic rhinosinusitis as COVID-19 protective factor?; Authors - Concepció Marin
		Autophagy; ATG4B/autophagin-1 regulates intestinal homeostasis and protects mice from experimental colitis.; Authors - Cabrera Sandra S
rat	IF	J Cell Biol; Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins.; Authors - Sebastian Kötter
human	WB,CoIP	Cell Rep; Analysis of proteome-wide degradation dynamics in ALS SOD1 iPSC-derived patient neurons reveals disrupted VCP homeostasis; Authors - Konstantinos Tsioras
human	WB	Phytomedicine; Ginsenoside Rg5 inhibits glioblastoma by activating ferroptosis via NR3C1/HSPB1/NCOA4; Authors - Guoqing Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (09-28-2020)

HEK293T lysate (10ug) ran on 4-20% gradient gel. Blocking in 5% BSA for 1 hour, followed by HSP27 (1:1000 in block) overnight at 4 degrees. Goat anti-rabbit HRP (1:10,000) for 1 hour in block.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

HSP27 Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:18284-1-AP)

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011