Anticorps Polyclonal de lapin anti-HSPA1L

• Phare
• Validé par KD/KO

HSPA1L Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 13970-1-AP

Synonymes

Heat shock 70 kDa protein 1L, HSP70 1L, HSP70 HOM, HSP70T, HSPA1L, hum70t

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Galerie de données de validation

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  WB analysis of mouse liver using 13970-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13970-1-AP (HSPA1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human saliva using 13970-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 13970-1-AP (HSPA1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13970-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13970-1-AP (HSPA1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13970-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13970-1-AP (HSPA1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13970-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13970-1-AP (HSPA1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 13970-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 13970-1-AP (HSPA1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 13970-1-AP

  IP result of anti-HSPA1L (IP:13970-1-AP, 4ug; Detection:13970-1-AP 1:1000) with HepG2 cells lysate 1800ug.

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  IHC staining of human endometrial cancer using 13970-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13970-1-AP (HSPA1L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human endometrial cancer using 13970-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13970-1-AP (HSPA1L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 13970-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13970-1-AP (HSPA1L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 13970-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13970-1-AP (HSPA1L antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 13970-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSPA1L antibody (13970-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HeLa, cellules HepG2, cellules MCF-7, tissu salivaire humain, tissu testiculaire de rat
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

13970-1-AP cible HSPA1L dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSPA1L Protéine recombinante Ag4995
• Nom complet: heat shock 70kDa protein 1-like
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC034483
• Symbole du gène: HSPA1L
• Identification du gène (NCBI): 3305
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPA1L (Hsp70-like protein 1, also known as HSP70-hom) is a member of Hsp70 family of heat shock proteins. Recently it has been reported that HSPA1L can interact with TLR4, activate dendritic cells and induce Th1 Response. This antibody well recognized the endogenous HSPA1L protein in multiple cell lines. (21730052)

Publications

Species	Application	Title
rat	WB	Front Endocrinol (Lausanne); Maternal Protein Restriction Induces Alterations in Hepatic Unfolded Protein Response-Related Molecules in Adult Rat Offspring.; Authors - Xiaomei Liu
human	WB	Virol J; Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9.; Authors - Yuting Yang
mouse	WB,IF	PeerJ; The heat shock protein family gene Hspa1l in male mice is dispensable for fertility.; Authors - Xin Wang ; KO Validated
mouse	IF	Neurobiol Dis; SBT-272 improves TDP-43 pathology in ALS upper motor neurons by modulating mitochondrial integrity, motility, and function; Authors - Mukesh Gautam
mouse	WB	J Cachexia Sarcopenia Muscle; The regulation of MFG-E8 on the mitophagy in diabetic sarcopenia via the HSPA1L-Parkin pathway and the effect of D-pinitol; Authors - Wenqian Zhao