Anticorps Polyclonal de lapin anti-HSPA4

• Phare
• Validé par KD/KO

HSPA4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 21206-1-AP

Synonymes

APG 2, APG2, Heat shock 70 kDa protein 4, heat shock 70kDa protein 4, HS24/P52, HSP70.4, hsp70RY, HSPA4, RY

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Galerie de données de validation

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  WB analysis using 21206-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21206-1-AP

  WB result of HSPA4 antibody (21206-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA4 transfected HEK-293 cells.

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  WB analysis of mouse testis using 21206-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 21206-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21206-1-AP

  IP Result of anti-HSPA4 (IP:21206-1-AP, 4ug; Detection:21206-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 21206-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 21206-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 21206-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 21206-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 21206-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 21206-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 21206-1-AP

  Immunofluorescent analysis of HepG2 cells, using HSPA4 antibody 21206-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of MCF-7 using 21206-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HSPA4 antibody (21206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules C6, cellules HEK-293, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du pancréas humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• ELISA: See 1 publications below

Informations sur le produit

21206-1-AP cible HSPA4 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSPA4 Protéine recombinante Ag15581
• Nom complet: heat shock 70kDa protein 4
• Masse moléculaire calculée: 840 aa, 94 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC110861
• Symbole du gène: HSPA4
• Identification du gène (NCBI): 3308
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPA4 (also known as Apg-2, HSP70RY) is a 110 kDa cytosolic protein, a member of the heat-shock protein 110 (HSP110) subfamily of HSP70 proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA4 is ubiquitously expressed and its expression is not heat inducible. Overexpression of HSPA4 has been reported in some leukemia and solid tumors. This antibody well recognized the endogenous HSPA4 protein in mouse brain/ testis tissues. (PMID: 21487003)

Publications

Species	Application	Title
mouse	WB	Int J Biochem Cell Biol; Folate deprivation induces cell cycle arrest at G0/G1 phase and apoptosis in hippocampal neuron cells through down-regulation of IGF-1 signaling pathway.; Authors - Yang Yang
human	ELISA	Eur J Clin Invest; Salivary monitoring related to major surgery.; Authors - Ullmann Yehuda Y
mouse	WB	Int J Med Sci; Exercise combined with trimetazidine improves anti-fatal stress capacity through enhancing autophagy and heat shock protein 70 of myocardium in mice.; Authors - Lingjun Jiang
human	WB	Mol Cell Proteomics; A new cellular interactome of SARS-CoV-2 nucleocapsid protein and its biological implications; Authors - Yuan-Qin Min