Anticorps Polyclonal de lapin anti-HSF4

• Phare
• Validé par KD/KO

HSF4 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 18797-1-AP

Synonymes

CTM, Heat shock factor protein 4, hHSF4, HSF 4, HSF4, HSTF 4

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Galerie de données de validation

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  WB analysis of human heart using 18797-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18797-1-AP (HSF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 18797-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18797-1-AP (HSF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 18797-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18797-1-AP (HSF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 18797-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18797-1-AP (HSF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, tissu cardiaque humain, tissu de muscle squelettique de souris, tissu de muscle squelettique humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below

Informations sur le produit

18797-1-AP cible HSF4 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: heat shock transcription factor 4
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 53-58 kDa
• Numéro d’acquisition GenBank: BC130383
• Symbole du gène: HSF4
• Identification du gène (NCBI): 3299
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSF4 belongs to the HSF family. HSF4 is a DNA-binding protein that specifically binds heat shock promoter elements (HSE). The isoform HSF4A represses transcription while the isoform HSF4B activates transcription. HSF4 is a homotrimer which exhibits constitutive DNA binding and forms trimers even in the absence of stress. It is expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues. Mutation of HSF4 will cause lamellar cataract which is known as Marner type cataract (CAM). Lamellar cataract is an autosomal dominant common type of infantile cataract. Finger malformation is observed in some kindreds. Inheritance of these forms of cataract is autosomal dominant.

Publications

Species	Application	Title
human	WB	Oncogene; FGD3 binds with HSF4 to suppress p65 expression and inhibit pancreatic cancer progression.; Authors - Feng Guo ; KD Validated
human	WB	Mol Cell Biochem; HSF4 promotes tumor progression of colorectal cancer by transactivating c-MET; Authors - Wenjing Zhang ; KD Validated
mouse	WB	Invest Ophthalmol Vis Sci; HSF4 Transcriptionally Activates Autophagy by Regulating ATG9a During Lens Terminal Differentiation; Authors - Jing Zhang