Anticorps Polyclonal de lapin anti-HSD3B7

HSD3B7 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 10488-1-AP

Synonymes

3 beta HSD VII, C(27) 3 beta HSD, HSD3B7, PFIC4, SDR11E3

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Galerie de données de validation

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  WB analysis of mouse liver using 10488-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10488-1-AP (HSD3B7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10488-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10488-1-AP (HSD3B7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10488-1-AP

  IP result of anti-HSD3B7 (IP:10488-1-AP, 4ug; Detection:10488-1-AP 1:300) with mouse liver tissue lysate 6000 ug.

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  IHC staining of human pancreas cancer using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human liver using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10488-1-AP (HSD3B7 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human kidney using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10488-1-AP (HSD3B7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10488-1-AP (HSD3B7 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human kidney using 10488-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10488-1-AP (HSD3B7 antibody) at dilution of 1:25 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

10488-1-AP cible HSD3B7 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSD3B7 Protéine recombinante Ag0755
• Nom complet: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC004929
• Symbole du gène: HSD3B7
• Identification du gène (NCBI): 80270
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSD3B7(3 beta-hydroxysteroid dehydrogenase type 7) is also named as 3-beta-HSD VII,C(27) 3-beta-HSD and belongs to the 3-beta-HSD family. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids and HSD VII is active against four 7-alpha-hydroxylated sterols. This enzyme is expressed predominantly in the liver(PMID:11067870). Defects in HSD3B7 are the cause of congenital bile acid synthesis defect type 1 (CBAS1). It has 2 isoforms produced by alternative splicing.