Anticorps Polyclonal de lapin anti-HSD3B2

HSD3B2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 15516-1-AP

Synonymes

3b HSD, HSD3B2, HSD3β2, HSDB, HSDB3, HSDB3B, SDR11E2

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Galerie de données de validation

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  WB analysis of mouse testis using 15516-1-AP

  mouse testis tissue was subjected to SDS PAGE followed by western blot with 15516-1-AP (HSD3B2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat testis using 15516-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15516-1-AP (HSD3B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 15516-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15516-1-AP (HSD3B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris,
• Résultats positifs en IHC: tissu testiculaire de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

15516-1-AP cible HSD3B2 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSD3B2 Protéine recombinante Ag7782
• Nom complet: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC038419
• Symbole du gène: HSD3B2
• Identification du gène (NCBI): 3284
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSD3B2(3-beta-hydroxy-Delta(5)-steroid dehydrogenase) belongs to the 3-beta-HSD family and catalyzes the oxidation and isomerization of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, thus leading to the formation of all classes of steroid hormones.HSD3B2 is expressed almost exclusively in adrenals and gonads, whereas HSD3B1 is expressed predominantly in placenta and skin (PMID:1682237). HSD3B2 plays a crucial role in steroid hormone biosynthesis and is thus of particular interest in hormone dependent tumors such as prostate cancer. Compared with normal tissue cytoplasmic HSD3B2 staining was stronger in prostate cancers (PMID: 29803408). Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2).

Publications

Species	Application	Title
mouse	WB	J Hazard Mater; Tributyltin chloride (TBTCL) induces cell injury via dysregulation of endoplasmic reticulum stress and autophagy in Leydig cells; Authors - Pengchen Chen
human	WB	Oncotarget; Generation and characteristics of human Sertoli cell line immortalized by overexpression of human telomerase.; Authors - Liping Wen
rat	WB, IHC	Endocrinology; A dietary medium-chain fatty acid, decanoic acid, inhibits recruitment of Nur77 to the HSD3B2 promoter in vitro, and reverses endocrine and metabolic abnormalities in a rat model of polycystic ovary syndrome.; Authors - Bao Hui Lee
mouse	IF	Front Physiol; Long-Term Maintenance and Meiotic Entry of Early Germ Cells in Murine Testicular Organoids Functionalized by 3D Printed Scaffolds and Air-Medium Interface Cultivation.; Authors - Guillaume Richer
mouse	IHC	Reprod Biomed Online; Brain-derived neurotrophic factor expression in granulosa lutein cells.; Authors - Dominguez Miguel A MA
human	IHC	Int J Clin Exp Pathol; Ectopic cortisol-producing adrenocortical adenoma in the renal hilum: histopathological features and steroidogenic enzyme profile.; Authors - Anli Tong