Anticorps Polyclonal de lapin anti-ERAB

• Phare
• Validé par KD/KO

ERAB Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10648-1-AP

Synonymes

17 beta HSD 10, 17b HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, HSD17B10, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, Type II HADH, XH98G2

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Galerie de données de validation

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  WB analysis of HeLa using 10648-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10648-1-AP

  WB result of ERAB antibody (10648-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERAB transfected HEK-293 cells.

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  WB analysis of HeLa using 10648-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10648-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10648-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 10648-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10648-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10648-1-AP

  IP result of anti-ERAB (IP:10648-1-AP, 4ug; Detection:10648-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human breast cancer using 10648-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10648-1-AP (ERAB antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A2780, cellules HEK-293, tissu cérébral humain, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IP: See 1 publications below

Informations sur le produit

10648-1-AP cible ERAB dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERAB Protéine recombinante Ag1020
• Nom complet: hydroxysteroid (17-beta) dehydrogenase 10
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC008708
• Symbole du gène: ERAB
• Identification du gène (NCBI): 3028
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSD17B10 (3-hydroxyacyl-CoA dehydrogenase type-2) is a multifunctional mitochondrial enzyme that acts on a wide spectrum of substrates, including neuroactive steroids, alcohols, leucine, and fatty acids, with a preference for short-chain methyl-branched acyl-CoAs(PMID:15860413).It has 2 isoforms produced by alternative splicing.Defects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) and mental retardation syndromic X-linked type 10 (MRXS10).

Publications

Species	Application	Title
mouse	WB	Cell Rep; Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.; Authors - Oliver Rackham
human	WB	Hum Mol Genet; Leber's hereditary optic neuropathy (LHON)-associated ND6 14 484 T > C mutation caused pleiotropic effects on the complex I, RNA homeostasis, apoptosis and mitophagy.; Authors - Min Liang
rat	WB	J Oleo Sci; Effects of Dietary Fat Restriction on Endurance Training-induced Metabolic Adaptations in Rat Skeletal Muscle.; Authors - Takuya Karasawa
mouse	WB	Circulation; Stimulates Short-Chain Dehydrogenase/Reductase Proteins to Alleviate Heart Failure Independent of Mitochondrial Protein Deacetylation; Authors - Matthew A Walker
rat	WB	Gen Comp Endocrinol; Testosterone regulates thymic remodeling by altering metabolic reprogramming in male rats; Authors - Dong Li
human,mouse	WB,IP	Phytother Res; Astragaloside IV protects renal tubular epithelial cells against oxidative stress-induced injury by upregulating CPT1A-mediated HSD17B10 lysine succinylation in diabetic kidney disease; Authors - Meng Wang