Anticorps Polyclonal de lapin anti-HSD17B1

HSD17B1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 25334-1-AP

Synonymes

17 beta HSD 1, 20 alpha HSD, E17KSR, E2DH, EDH17B1, EDH17B2, EDHB17, HSD17, HSD17B1, SDR28C1

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Galerie de données de validation

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  WB analysis of human placenta using 25334-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25334-1-AP (HSD17B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 25334-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25334-1-AP (HSD17B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 25334-1-AP

  IP result of anti-HSD17B1 (IP:25334-1-AP, 4ug; Detection:25334-1-AP 1:500) with human placenta tissue lysate 2600 ug.

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  IHC staining of human placenta using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25334-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25334-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 25334-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25334-1-AP (HSD17B1 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain,
• Résultats positifs en IP: tissu placentaire humain,
• Résultats positifs en IHC: tissu placentaire humain, tissu de cancer de l'endomètre humain, tissu de cancer du côlon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

25334-1-AP cible HSD17B1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSD17B1 Protéine recombinante Ag17886
• Nom complet: hydroxysteroid (17-beta) dehydrogenase 1
• Masse moléculaire calculée: 328 aa, 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC104752
• Symbole du gène: HSD17B1
• Identification du gène (NCBI): 3292
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Transl Med; An integrated approach of network pharmacology, molecular docking, and experimental verification uncovers kaempferol as the effective modulator of HSD17B1 for treatment of endometrial cancer; Authors - Guan-Yu Ruan
mouse	WB	Cells; HDAC1-Mediated lncRNA Stimulatory Factor of Follicular Development to Inhibit the Apoptosis of Granulosa Cells and Regulate Sexual Maturity through miR-202-3p-COX1 Axis; Authors - Xiaofeng Zhou
mouse	WB	J Nanobiotechnology; Ti3C2 nanosheet-induced autophagy derails ovarian functions; Authors - Limei Yang