Anticorps Polyclonal de lapin anti-HSD11B2

HSD11B2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 14192-1-AP

Synonymes

11 beta HSD2, 11 DH2, AME, AME1, HSD11B2, HSD11K, HSD2, SDR9C3

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Galerie de données de validation

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  WB analysis using 14192-1-AP

  Human placenta tissue lysates were subjected to SDS PAGE followed by western blot with 14192-1-AP (HSD11B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14192-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14192-1-AP (HSD11B2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 14192-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14192-1-AP (HSD11B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 14192-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14192-1-AP (HSD11B2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14192-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14192-1-AP (HSD11B2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14192-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14192-1-AP (HSD11B2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules HEK-293 transfectées, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

14192-1-AP cible HSD11B2 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSD11B2 Protéine recombinante Ag5146
• Nom complet: hydroxysteroid (11-beta) dehydrogenase 2
• Masse moléculaire calculée: 405 aa, 44 kDa
• Poids moléculaire observé: 40-43 kDa
• Numéro d’acquisition GenBank: BC036780
• Symbole du gène: HSD11B2
• Identification du gène (NCBI): 3291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The HSD11B2 gene encodes the type II isoform of 11-beta-hydroxysteroid dehydrogenase, a microsomal enzyme complex responsible for the interconversion of biologically active cortisol and inactive cortisone. This protein catalyzes the cortisol-to-cortisone reaction. This protein expresses approximately 44 kDa in placenta, trophoblast, and distal colon, but in kidney tissue, there are two bands of approximately 44 and 48 kDa can be consistently observed and no signal is seen in decidua, adrenal, or liver(PMID:9048640). HSD11B2 protein was detected in CEM-C7 cells via immunoblot analysis as a dimer and trimer of approximately 80 and 120 kDa (PMID:23762608).

Publications

Species	Application	Title
rat	WB	Nutrients; Maternal High-Fructose Corn Syrup Intake Impairs Corticosterone Clearance by Reducing Renal 11β-Hsd2 Activity via miR-27a-Mediated Mechanism in Rat Offspring; Authors - Yuki Nouchi
mouse	WB	Int Immunopharmacol; Mineralocorticoid promotes intestinal inflammation through receptor dependent IL17 production in ILC3s; Authors - Rongchuan Zhao
rat	WB	Oncotarget; Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats.; Authors - Shuai Lian
mouse	WB	Ecotoxicol Environ Saf; Heat stress affects fetal brain and intestinal function associated with the alterations of placental barrier in late pregnant mouse.; Authors - Huiduo Guo
human	WB,IHC	Carcinogenesis; Modulation of the oral glucocorticoid system during black raspberry mediated oral cancer chemoprevention.; Authors - Divya Nedungadi ; KD Validated
pig	WB	Sci Rep; Proteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets.; Authors - Haodong Chen